Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute for Agricultural and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute for Agricultural and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Institutes of Green Bio Science & Technology, Seoul National University, Pyeongchang-gun, Gangwon-do 25354, Republic of Korea.
Food Res Int. 2021 Mar;141:110105. doi: 10.1016/j.foodres.2021.110105. Epub 2021 Jan 5.
We investigated the bactericidal effects against Escherichia coli O157:H7 of light-emitting diodes (LEDs) emitting blue light of four different peak wavelengths ranging from 395 to 425 nm in water. Furthermore, we investigated inactivation in the presence of reactive oxygen species (ROS) scavengers to elucidate the contribution of bacterial inactivation. An aluminum chamber was constructed and coated in carbon to block exterior light, and a single blue light LED with a rear heat sink was attached to the chamber lid. Effective inactivation of the pathogen was observed for all blue light LED irradiation at 305, 405, 415, and 425 nm. The log-linear with shoulder and tail model and log-linear model described the survival of the bacteria after blue light LED treatments. Not just the effects of ROS but also photophysical effects were shown with the addition of mannitol, a ROS scavenger. The integrity of the cell membrane was damaged regardless of the presence of ROS, which indicates that photophysical effects were sufficient to induce damage to the cell membrane. In addition, activity of succinate-coenzyme Q reductase, which participates in respiratory metabolism to generate energy, decreased in the absence of ROS and decreased further in the presence of ROS.
我们研究了从 395 到 425nm 的四个不同峰值波长的蓝光发光二极管（LED）对水中大肠杆菌 O157:H7 的杀菌效果。此外，我们还研究了在活性氧（ROS）清除剂存在下的失活动力学，以阐明细菌失活的贡献。构建了一个涂有碳的铝室以阻挡外部光线，并将单个带有后置散热器的蓝光 LED 安装在腔室盖上。在所有的蓝光 LED 辐照下，305nm、405nm、415nm 和 425nm 均能有效杀灭病原体。对数线性加肩尾模型和对数线性模型描述了蓝光 LED 处理后细菌的存活情况。添加 ROS 清除剂甘露醇不仅显示了 ROS 的作用，还显示了光物理作用。细胞膜的完整性受到了破坏，而不管 ROS 是否存在，这表明光物理作用足以对细胞膜造成损伤。此外，参与呼吸代谢以产生能量的琥珀酸辅酶 Q 还原酶的活性在没有 ROS 的情况下降低，而在有 ROS 的情况下进一步降低。
