School of Life Science, Nanchang University, Nanchang, China.
National Engineering Research Center for Bioengineering Drugs And The Technologies, Institute of Translational Medicine, Nanchang University, Nanchang, China.
Dis Markers. 2022 May 18;2022:8762936. doi: 10.1155/2022/8762936. eCollection 2022.
Airborne diseases are transmitted by pathogens in the air. The complex microbial environment in wards is usually considered a major cause of nosocomial infection of various diseases which greatly influences the health of patients with chronic diseases, whereas the illuminant of wards impacts on the microbe especially the disease marker strain is seldom studied. In the present study, high-throughput sequencing was used to study the effect of yellow light on airborne microbial composition, and changes of transcriptome of marker strains , and , which were isolated from wards, were further studied after the irradiation by yellow light. High-throughput sequencing results indicated that yellow light significantly decreased -diversity. The relative abundance of Firmicutes at the phylum level, and o , at the genus level were significantly reduced. RNA sequencing results declared that yellow light significantly downregulated the genes associated with flagella, heme transport system and carbohydrate, amino acid metabolism in , and the genes related to arginine biosynthesis and the biosynthesis of isoleucine, leucine, and valine in . Meanwhile, yellow light significantly upregulated the genes relating to porphyrin metabolism . In conclusion, our work reveals the impacts of yellow light on the microbe in wards, pointing out the application value of yellow light in the prevention of infectious diseases in clinical practice.
空气传播疾病是由空气中的病原体传播的。病房中复杂的微生物环境通常被认为是导致各种疾病院内感染的主要原因，这极大地影响了慢性病患者的健康，而病房的照明对微生物的影响，尤其是对疾病标志物菌株的影响，却很少被研究。在本研究中，我们使用高通量测序来研究黄光对空气传播微生物组成的影响，并进一步研究了从病房中分离出的标记菌株、和在黄光照射后的转录组变化。高通量测序结果表明，黄光显著降低了 -多样性。厚壁菌门水平的相对丰度和属水平的 o 显著降低。RNA 测序结果表明，黄光显著下调了与鞭毛、血红素转运系统和碳水化合物、氨基酸代谢相关的基因，以及与精氨酸生物合成和异亮氨酸、亮氨酸和缬氨酸生物合成相关的基因在 中。同时，黄光显著上调了与卟啉代谢相关的基因。综上所述，我们的工作揭示了黄光对病房中微生物的影响，指出了黄光在临床实践中预防传染病的应用价值。
