Premedical Science, College of Medicine, Chosun University, Republic of Korea.
Department of Laboratory Medicine, College of Medicine, Chosun University, Republic of Korea.
Jpn J Infect Dis. 2021 Nov 22;74(6):499-506. doi: 10.7883/yoken.JJID.2020.765. Epub 2021 Feb 26.
Various mechanisms underlying antimicrobial resistance in Acinetobacter baumannii have been reported. However, the relationships between efflux pump activity, biofilm formation, and antimicrobial resistance in A. baumannii is controversial. In this study, we investigated the relative expression of RND efflux pump genes, H33342 efflux activity, and biofilm-forming activity in 120 A. baumannii clinical isolates, examined their potential relationships with each other, and statistically analyzed their effects on antibiotic resistance. High adeB expression and high H33342 efflux activity were correlated with low biofilm-forming activity. High adeB expression was significantly correlated with resistance to tigecycline and cefotaxime, but not with the multidrug resistance (MDR) phenotype. Importantly, only high adeJ expression was significantly correlated with the MDR phenotype and was correlated with resistance to various antibiotics. However, we found no significant correlation between adeJ expression and biofilm-forming activity. Furthermore, adeG expression was not correlated with antibiotic resistance and biofilm-forming activity. The results of multivariate analysis showed that adeB overexpression and high H33342 efflux activity were related to biofilm-forming activity, and only adeJ overexpression was significantly associated with the MDR phenotype, highlighting the importance of adeJ overexpression.
已经报道了鲍曼不动杆菌产生抗微生物药物耐药性的多种机制。然而，鲍曼不动杆菌中外排泵活性、生物膜形成和抗微生物药物耐药性之间的关系存在争议。在本研究中，我们调查了 120 株鲍曼不动杆菌临床分离株中 RND 外排泵基因的相对表达、H33342 外排活性和生物膜形成活性，研究了它们之间的潜在关系，并对它们对抗生素耐药性的影响进行了统计学分析。高 adeB 表达和高 H33342 外排活性与低生物膜形成活性相关。高 adeB 表达与对替加环素和头孢噻肟的耐药性显著相关，但与多药耐药（MDR）表型无关。重要的是，只有高 adeJ 表达与 MDR 表型显著相关，并与对各种抗生素的耐药性相关。然而，我们没有发现 adeJ 表达与生物膜形成活性之间存在显著相关性。此外，adeG 表达与抗生素耐药性和生物膜形成活性无关。多变量分析的结果表明，adeB 过表达和高 H33342 外排活性与生物膜形成活性有关，只有 adeJ 过表达与 MDR 表型显著相关，突出了 adeJ 过表达的重要性。
