Hashimoto M, Hata R, Tyuma I, Isomoto A, Uozumi M
Department of Physico-chemical Physiology, Osaka University Medical School, Japan.
Adv Exp Med Biol. 1988;222:231-8. doi: 10.1007/978-1-4615-9510-6_27.
A method for quantitative analysis of hemoglobin color to estimate the oxygen saturation was developed. The method uses an image-input and -processing system composed of a 3-tube video camera and a digital image analyzer. Using the system connected to a microscope, facilitated diffusion of oxygen in hemoglobin solutions was observed and analyzed in a position-sensitive way. The results confirmed its applicability to this study and gave information about the diffusion mechanism expressed by the empirical formula J = kY, where J is the flux of oxygen, Y is the oxygen saturation of hemoglobin, and k is a constant.
开发了一种通过血红蛋白颜色定量分析来估计氧饱和度的方法。该方法使用由三管摄像机和数字图像分析仪组成的图像输入与处理系统。利用连接到显微镜的该系统,以位置敏感的方式观察和分析了氧在血红蛋白溶液中的易化扩散。结果证实了其在本研究中的适用性,并给出了由经验公式J = kY表示的扩散机制的相关信息,其中J是氧通量,Y是血红蛋白的氧饱和度,k是一个常数。