大鼠脑谱的测量。

Measurement of rat brain spectra.

作者信息

Hagihara B, Okutani N, Nishioka M, Negayama N, Ohtani W, Takamura S, Oka K

机构信息

Hagihara Medical Technology Research Institute, Hyogo, Japan.

出版信息

Adv Exp Med Biol. 1988;222:351-7. doi: 10.1007/978-1-4615-9510-6_41.

DOI:10.1007/978-1-4615-9510-6_41

PMID:3364258

Abstract

A method of optically monitoring oxygen saturation of blood haemoglobin in the rat brain stem was developed. Optical fiber bundles were attached to the orifices of both ears of the rat to irradiate incident light from one ear and receive transmitted light from the other ear. Absorption spectra were measured using a white-light source and a photodiode array spectrophotometer. Stable measurements of optical time courses and absorption spectra were made using this "ear-to-ear" path. Oxygen saturation levels were calculated from spectra by using a suitable reference and an improved method of determining spectral peak heights.

摘要

开发了一种光学监测大鼠脑干中血红蛋白血氧饱和度的方法。将光纤束连接到大鼠双耳的孔口，以从一只耳朵照射入射光，并从另一只耳朵接收透射光。使用白光源和光电二极管阵列分光光度计测量吸收光谱。利用这种“耳到耳”路径对光学时间进程和吸收光谱进行了稳定测量。通过使用合适的参考物和一种改进的确定光谱峰高的方法，从光谱中计算出血氧饱和度水平。