Amelink Arjen, Christiaanse Theo, Sterenborg Henricus J C M
Department of Radiation Oncology, Center for Optical Diagnostics and Therapy, Erasmus MC, P.O. Box 2040, 3000 CA Rotterdam, The Netherlands.
Opt Lett. 2009 May 15;34(10):1525-7. doi: 10.1364/ol.34.001525.
Fiber-optic reflectance measurements were performed on scattering phantoms containing red blood cells. The oxygen pressure in the phantom was decreased by nitrogen gas bubbling, while carefully controlling and monitoring the temperature (37 degrees C) and pH (7.4). The blood oxygen saturation (SO(2)) was extracted from the optical spectroscopic measurements by fitting the spectra over the wavelength range of 350-850 nm to a model that includes the effects of pigment packaging. The effect of using different oxy- and deoxyhemoglobin extinction spectra on the quality and accuracy of the fits is analyzed. The optically extracted SO(2) is compared with the standard oxygen dissociation curve (ODC). Depending on the choice of hemoglobin extinction spectra the absolute deviation between the ODC and the optically extracted SO(2) is less than 2.5% over the measured range of saturations (4%-99%).
对含有红细胞的散射体模进行了光纤反射率测量。通过鼓入氮气降低体模中的氧压，同时仔细控制和监测温度（37摄氏度）和pH值（7.4）。通过将350 - 850纳米波长范围内的光谱拟合到一个包含色素包装效应的模型，从光学光谱测量中提取血氧饱和度（SO₂）。分析了使用不同的氧合血红蛋白和脱氧血红蛋白消光光谱对拟合质量和准确性的影响。将光学提取的SO₂与标准氧解离曲线（ODC）进行比较。根据血红蛋白消光光谱的选择，在测量的饱和度范围（4% - 99%）内，ODC与光学提取的SO₂之间的绝对偏差小于2.5%。
