Husnurrizal Husnurrizal, Siregar Tongku Nizwan, Gholib Gholib, Panjaitan Budianto, Armansyah Teuku, Wahyuni Sri
Laboratory of Reproduction, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.
Laboratory of Physiology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.
Vet World. 2021 Jan;14(1):230-236. doi: 10.14202/vetworld.2021.230-236. Epub 2021 Jan 26.
This study aimed at determining the profiles of progesterone and bovine interferon-τ (bIFN-τ) and the correlation between the two in repeat breeding (RB) Aceh cattle and non-RB Aceh cattle.
The study was performed on five RB and five non-RB Aceh cows. These cows were subjected to estrous synchronization using the prostaglandin F2 alpha hormone, which was followed by artificial insemination (AI). Serum samples were collected on days 5, 6, 7, 15, 16, and 17 after AI to measure the concentration of progesterone at the beginning and end of the luteal phase and from days 14 to 18 after AI to measure the concentration of bIFN-τ. The concentrations of progesterone and bIFN-τ were determined using enzyme-linked immunosorbent assay. Pregnancy examinations were performed by ultrasonography on days 25, 35, 45, and 55 after AI. Data for progesterone and bIFN-τ concentrations were analyzed using the Mann-Whitney and t-tests, and the correlation between progesterone and bIFN-τ was analyzed using the Spearman correlation test.
The average concentration of progesterone in RB Aceh cows tended to be lower than non-RB Aceh cows, but it was not significantly different (p>0.05). Similar results also found in the concentration of bIFN-τ which RB Aceh cows tended to have lower bIFN-τ concentrations compared to non-RB Aceh cows, but it was also not significantly different (p>0.05). Moreover, the concentrations of progesterone and bIFN-τ in RB and non-RB Aceh cows did not show a significant correlation (p>0.05). These results of the ultrasonography showed that non-RB Aceh cows were pregnant from day 25 to day 55 after AI, whereas RB Aceh cows were not pregnant and had early embryonic death.
The concentrations of progesterone and bIFN-τ in non-RB Aceh cows tended to be higher than those in RB Aceh cows, although, it was not significantly different. Non-RB Aceh cows were able to maintain pregnancy until day 55, whereas RB Aceh cows were diagnosed with early embryonic death before day 25 after AI.
本研究旨在确定亚齐牛重复配种（RB）和非重复配种亚齐牛的孕酮和牛干扰素-τ（bIFN-τ）水平及其二者之间的相关性。
本研究选取了5头重复配种亚齐牛和5头非重复配种亚齐牛。这些母牛使用前列腺素F2α激素进行发情同步，随后进行人工授精（AI）。在人工授精后的第5、6、7、15、16和17天采集血清样本，以测量黄体期开始和结束时的孕酮浓度，并在人工授精后的第14至18天采集样本以测量bIFN-τ的浓度。使用酶联免疫吸附测定法测定孕酮和bIFN-τ的浓度。在人工授精后的第25、35、45和55天通过超声检查进行妊娠检测。使用Mann-Whitney检验和t检验分析孕酮和bIFN-τ浓度的数据，并使用Spearman相关检验分析孕酮和bIFN-τ之间的相关性。
亚齐牛重复配种母牛的孕酮平均浓度往往低于非重复配种亚齐牛，但差异不显著（p>0.05）。在bIFN-τ浓度方面也发现了类似结果，即亚齐牛重复配种母牛的bIFN-τ浓度往往低于非重复配种亚齐牛，但同样差异不显著（p>0.05）。此外，亚齐牛重复配种和非重复配种母牛的孕酮和bIFN-τ浓度之间没有显著相关性（p>0.05）。超声检查结果表明，非重复配种亚齐牛在人工授精后第25天至第55天怀孕，而亚齐牛重复配种母牛未怀孕且发生了早期胚胎死亡。
非重复配种亚齐牛的孕酮和bIFN-τ浓度往往高于亚齐牛重复配种母牛，尽管差异不显著。非重复配种亚齐牛能够维持妊娠至第55天，而亚齐牛重复配种母牛在人工授精后第25天前被诊断为早期胚胎死亡。
