The Effect of Infection on CD86 Expression in THP-1 Cells.

BACKGROUND

() is a destructive opportunistic dimorphic fungal which can cause lethiferous Talaromycosis, but the clearance of mainly depends on the innate immune response.

OBJECTIVE

To investigate whether can inhibit the expression of CD86 in THP-1 cells after infection and discuss the potential mechanisms.

METHODS

Western blot and immunoelectron microscopy were used to detect the CD86 expression on cultured on BHI medium at 37°C. Western blot, enzyme-linked immunoassay and immunofluorescence were used to detect the change of CD86 expression on macrophages incubating with . Enzyme-linked immunoassay was used to detect the content of CD86 in supernatant in the co-culture system. Immunohistochemistry and immunoelectron microscopy were used to detect the expression of CD86 on incubating with macrophages.

RESULTS

did not express CD86 when cultured separately at 37°C detected by Western blot and immunoelectron microscopy, but it did express CD86 when incubated with macrophages detected by immunohistochemistry and immunoelectron microscopy. The CD86 expression of macrophages significantly decreased at 72 hours when infected with while the content of CD86 in supernatant significantly increased at 72 hours compared with the control group which were detected by Western blot, enzyme-linked immunoassay and immunofluorescence.

CONCLUSION

1. After infection, CD86 expression on THP-1 decreased, and with the progression of infection, insufficient polarization of M1 macrophages gradually appeared; 2) may adsorb or uptake CD86 in supernatant produced by macrophages during the contact with THP-1 cells, thus leading to the consumption of CD86 in macrophages.