Loshali Aanchal, Joshi Bhuwan Chandra, Sundriyal Ankush, Uniyal Sushmita
School of Pharmaceutical Sciences, Sardar Bhagwan Singh Post Graduate Institute of Biomedical Sciences &Research, Balawala, Dehradun, Uttarakhand, 248001, India.
School of Pharmaceutical Sciences, Shri Guru Ram Rai Institute of Technology & Science, Patel Nagar, Dehradun, Uttarakhand, 248001, India.
Heliyon. 2021 Feb 5;7(2):e06195. doi: 10.1016/j.heliyon.2021.e06195. eCollection 2021 Feb.
Linn. (Urticaceae) is a medicinal plant that has shown various therapeutic utilities in folklore medicine along with its use in the treatment of epilepsy. The entire plant has a sensible reservoir of nutritional elements and micronutrients. The purpose of the present study was to investigate the antiepileptic effect of antioxidant potent extract of root on animal models. Antioxidant activity of various solvent extracts i.e. Petroleum ether extract (PEE), Ethyl acetate extract (EAE), Chloroform extract (CE) and Ethanolic extract (EE) were screened by DPPH radical scavenging assay using Ascorbic acid as the standard. Further the most potent antioxidant extract was subjected to antiepileptic activity against MES and PTZ model. The IC values of different extracts (PEE, CE, EAE, and EE) in antioxidant assay were found to be 167.54 ± 1.97, 134.41 ± 0.82, 88.15 ± 1.39 and 186.38 ± 1.91 μg/ml in DPPH radical scavenging assay, respectively. The EAE has showed the potent antioxidant activity. In experimental study the EAE (100 and 200 mg/kg, p.o) has found to be effective and significant against MES and PTZ induced seizures. The present study also suggested that antioxidant potent extract (EAE) of root has antiepileptic effect against MES and PTZ induced seizures. However, further research studies will investigate the active component(s) of responsible for the observed anticonvulsant effects.
（荨麻科）是一种药用植物，在民间医学中显示出多种治疗用途，也用于治疗癫痫。整株植物含有丰富的营养元素和微量营养素。本研究的目的是研究该植物根部具有抗氧化活性的提取物对动物模型的抗癫痫作用。通过以抗坏血酸为标准的DPPH自由基清除试验筛选了各种溶剂提取物即石油醚提取物（PEE）、乙酸乙酯提取物（EAE）、氯仿提取物（CE）和乙醇提取物（EE）的抗氧化活性。进一步对最具抗氧化活性的提取物进行了针对最大电休克（MES）和戊四氮（PTZ）模型的抗癫痫活性研究。在DPPH自由基清除试验中，不同提取物（PEE、CE、EAE和EE）的IC值分别为167.54±1.97、134.41±0.82、88.15±1.39和186.38±1.91μg/ml。EAE显示出强大的抗氧化活性。在实验研究中，EAE（100和200mg/kg，口服）对MES和PTZ诱导的癫痫发作有效且具有显著性。本研究还表明，该植物根部具有抗氧化活性的提取物（EAE）对MES和PTZ诱导的癫痫发作具有抗癫痫作用。然而，进一步的研究将调查导致观察到的抗惊厥作用的活性成分。
