Pietkiewicz Jadwiga, Danielewicz Regina, Wandzel Czesław, Beznosiuk Jarosław, Szuba Andrzej, Samsel-Czekała Małgorzata, Gamian Andrzej
Department of Biochemistry, Wroclaw Medical University, Wroclaw, Chalubinskiego 10, 50-368 Wroclaw, Poland.
Metanel Group Spółka Akcyjna, Nad Bialką 2A, 43-503 Czechowice-Dziedzice, Poland.
ACS Omega. 2021 Feb 1;6(6):4255-4261. doi: 10.1021/acsomega.0c05199. eCollection 2021 Feb 16.
Enolase is a conservative protein. Its cellular enzymatic activity catalyzes the conversion of 2-phospho-d-glycerate (2-PGA) to a phosphoenolpyruvate (PEP) product in the glycolysis pathway. This enzyme also has a multifunctional nature participating in several biological processes. This work aims to determine the effect of water polarization on the catalytic activity of enolase. The experiments have been set based on the concept that water, a polar dielectric, may undergo the phenomenon of electric polarization, decreasing its configurational and vibrational entropy. Prior to the reaction, the 2-PGA substrate was incubated for 5 h in the glass cuvette with an attached chip-inductor. The latter device was designed to transfer quantum information about a given quantum state from the quantum state generator to water by a phonon resonance. Then, such substrate samples preincubated with the chip-inductor were removed every hour in a separate quartz cuvette with the enzyme to determine its catalytic activity. The influence of the chip-inductor on the preincubated substrate resulted in an increase in the catalytic activity of enolase by 30% compared to the control substrate, not preincubated with the chip-inductor. This suggests that the catalytic activity of the enzyme is augmented when the substrate was primed by chip-inductors. In another kind of experiment, wherein enolase was exposed to methylglyoxal modification, the catalytic activity of the enzyme dropped to 71.7%, while the same enzyme glycated with methylglyoxal primed by chip-inductors restored its activity by 8.4%. This shows the protective effect of chip-inductors on enolase activity despite the harmful effect of methylglyoxal on the protein.
烯醇化酶是一种保守蛋白。其细胞酶活性在糖酵解途径中催化2-磷酸-D-甘油酸（2-PGA）转化为磷酸烯醇丙酮酸（PEP）产物。这种酶还具有多功能性，参与多种生物过程。这项工作旨在确定水极化对烯醇化酶催化活性的影响。实验基于这样一个概念进行设置，即水作为一种极性电介质，可能会发生电极化现象，从而降低其构型熵和振动熵。在反应之前，将2-PGA底物在带有附着芯片电感的玻璃比色皿中孵育5小时。后一种装置设计用于通过声子共振将关于给定量子态的量子信息从量子态发生器传递到水中。然后，每隔一小时将与芯片电感预孵育的底物样品转移到一个单独的装有酶的石英比色皿中，以测定其催化活性。与未用芯片电感预孵育的对照底物相比，芯片电感对预孵育底物的影响导致烯醇化酶的催化活性提高了30%。这表明当底物用芯片电感预处理时，酶的催化活性增强。在另一类实验中，烯醇化酶经甲基乙二醛修饰后，酶的催化活性降至71.7%，而同样用芯片电感预处理过的经甲基乙二醛糖基化的该酶，其活性恢复了8.4%。这表明尽管甲基乙二醛对蛋白质有有害影响，但芯片电感对烯醇化酶活性具有保护作用。
