O Connor Kelly, Cho Sung Bin, Chung Hye Jin
Department of Dermatology, Boston University School of Medicine, Boston, Massachusetts.
Yonsei Seran Dermatology and Laser Clinic, Seoul, South Korea.
Lasers Surg Med. 2021 Oct;53(8):1059-1064. doi: 10.1002/lsm.23390. Epub 2021 Feb 28.
The aim of this study is to histologically characterize the wound healing process of in vivo human skin treated with 1064- and 532-nm microlens array (MLA)-type picosecond lasers.
STUDY DESIGN/MATERIALS AND METHODS: Three patients (Fitzpatrick skin types II-IV), who were undergoing future cosmetic abdominoplasties, were treated with 1064- and 532-nm MLA-type lasers under different fluence settings. Treatments were performed 2 weeks, 1 week, and immediately prior to surgery. Skin samples were harvested from the resected tissue with 8 mm punch biopsies immediately after the abdominoplasties were performed.
The study demonstrates that intraepidermal vacuoles, created from tissue damage induced by the laser, are histologically resolved within 1 week without persistent damage to the dermoepidermal junction or vasculature. After 2 weeks, all foci of microscopic epidermal necrotic debris had either resolved or migrated to more superficial levels in the stratum corneum. There was no evidence of persistent vascular damage, increased melanophages, or accumulation of melanin in the dermis at 2 weeks. Furthermore, the 1064-nm picosecond laser with the high fluence setting demonstrated the capacity to fractionally ablate the epidermis and induce multifocal fibrosis in the papillary dermis in lighter skin types.
This is the first study to demonstrate the wound healing profile of in vivo human skin after treatment with the picosecond 1064- and 532-nm MLA-type lasers. It shows that laser-induced tissue damage is histologically resolved within 2 weeks, clinically reflecting a favorable safety profile and short downtime. The study also shows that the picosecond laser can be used to induce either fractional ablative or non-ablative effects, depending on the fluence settings used. Lasers Surg. Med. © 2021 Wiley Periodicals LLC.
本研究旨在从组织学角度描述用1064纳米和532纳米微透镜阵列（MLA）型皮秒激光治疗的体内人体皮肤伤口愈合过程。
研究设计/材料与方法：三名患者（菲茨帕特里克皮肤分型为II - IV型），计划进行腹部整形美容手术，在不同能量密度设置下用1064纳米和532纳米MLA型激光进行治疗。治疗分别在手术前2周、1周和即刻进行。腹部整形手术后立即用8毫米打孔活检从切除组织中获取皮肤样本。
研究表明，由激光诱导的组织损伤产生的表皮内空泡在1周内从组织学上得到解决，而对真皮表皮连接或脉管系统无持续性损伤。2周后，所有微观表皮坏死碎片灶要么已消退，要么已迁移至角质层更浅表的层面。2周时没有证据表明存在持续性血管损伤、黑素巨噬细胞增加或真皮中黑色素积聚。此外，高能量密度设置的1064纳米皮秒激光显示出在较浅肤色类型中能够部分消融表皮并在乳头层真皮中诱导多灶性纤维化。
这是第一项展示用皮秒1064纳米和532纳米MLA型激光治疗后体内人体皮肤伤口愈合情况的研究。研究表明激光诱导的组织损伤在2周内从组织学上得到解决，临床反映出良好的安全性和短的停工期。该研究还表明，根据所使用的能量密度设置，皮秒激光可用于诱导部分剥脱或非剥脱效应。《激光外科与医学》。©2021威利期刊有限责任公司。
