Zheng Shu-Ze, Guo Qiang, Zhang Gui-Min, Pan Li-Hong, Zeng Ke-Wu
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Beijing 100191, China.
State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine, Lunan Pharmaceutical Group Co., Ltd. Linyi 276006, China.
Zhongguo Zhong Yao Za Zhi. 2021 Feb;46(3):520-525. doi: 10.19540/j.cnki.cjcmm.20201125.401.
Shouhui Tongbian Capsules was used to explore the therapeutic effect and potential mechanism on slow transit constipation model mice induced by loperamide hydrochloride. In the experiment, loperamide hydrochloride-induced ICR mice were used as the model of slow transit constipation. Fifty ICR mice were divided into the blank group, model group and high, medium and low dose groups of Shouhui Tongbian Capsules extract(100, 200 and 400 mg·kg~(-1)). The model group and the administration groups were then modeled using loperamide hydrochloride intragastrically to obtain slow transit constipation. After successful modeling, high, medium and low doses of drugs were given to each drug group by intragastric administration. After 14 days of administration, the first defecation time, 6 h defecation grain number, 6 h defecation wet weight and dry weight, black feces discharged within 6 h and the fecal water content were measured. Intestinal tissues were taken for c-Kit and SCF immunohistochemical sections to detect the expression of c-Kit and SCF in the blank group, model group and high, medium and low dose groups of the medicinal extract of Shouhui Tongbian Capsules. The tissue changes in the intestinal wall of mice were detected by HE staining. At the same time, partial intestinal tissues were taken to test the activity of ATP synthase and isocitrate dehydrogenase in intestinal tissues of mice. RESULTS:: showed that Shouhui Tongbian Capsules effectively improved the symptoms of slow transit constipation in ICR mice and promoted intestinal movement. Shouhui Tongbian Capsules obviously shortened the time of discharging black stool for the first time, improved the intestinal propulsion rate, increased the water content and amount of feces, and improved the constipation symptoms. Mechanism study revealed that Shouhui Tongbian Capsules increased ATP synthase activity and mitochondrial isocitrate dehydrogenase activity in intestinal tissue, and up-regulated c-Kit/SCF signaling pathway to promote interstitial Cajal cells proliferation, intestinal nerve transmission, intestinal motility and transport capacity.
寿辉通便胶囊用于探讨其对盐酸洛哌丁胺诱导的慢传输型便秘模型小鼠的治疗作用及潜在机制。实验中，将盐酸洛哌丁胺诱导的ICR小鼠作为慢传输型便秘模型。50只ICR小鼠分为空白组、模型组以及寿辉通便胶囊提取物高、中、低剂量组（100、200和400mg·kg⁻¹）。然后对模型组和给药组小鼠进行盐酸洛哌丁胺灌胃造模，以获得慢传输型便秘。造模成功后，对各给药组分别进行高、中、低剂量药物灌胃给药。给药14天后，测量首次排便时间、6小时排便粒数、6小时排便湿重和干重、6小时内排出的黑便量以及粪便含水量。取肠道组织进行c-Kit和SCF免疫组化切片，检测寿辉通便胶囊药物提取物空白组、模型组以及高、中、低剂量组中c-Kit和SCF的表达。通过HE染色检测小鼠肠壁的组织变化。同时，取部分肠道组织检测小鼠肠道组织中ATP合酶和异柠檬酸脱氢酶的活性。结果显示：寿辉通便胶囊有效改善了ICR小鼠慢传输型便秘症状，促进了肠道蠕动。寿辉通便胶囊明显缩短了首次排出黑便的时间，提高了肠道推进率，增加了粪便含水量和量，改善了便秘症状。机制研究表明，寿辉通便胶囊增加了肠道组织中ATP合酶活性和线粒体异柠檬酸脱氢酶活性，上调了c-Kit/SCF信号通路，以促进间质 Cajal 细胞增殖、肠道神经传导、肠道蠕动和运输能力。
