Guo Yanan, Song Liqiong, Huang Yuanming, Li Xianping, Xiao Yuchun, Wang Zhihuan, Ren Zhihong
State Key Laboratory for Infectious Disease Prevention and Control, Research Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Chinese Academy of Medical Sciences, Beijing, China.
Front Nutr. 2023 Jan 5;9:1039403. doi: 10.3389/fnut.2022.1039403. eCollection 2022.
Slow transit constipation (STC) is a common disorder in the digestive system. This study aimed to evaluate the effects of stachyose (ST) and Furu 2019 () alone or combined on diphenoxylate-induced constipation and explore the underlying mechanisms using a mouse model.
ICR mice were randomly divided into five groups. The normal and constipation model groups were intragastrically administrated with PBS. The ST, , and synbiotic groups were intragastrically administrated with ST (1.5 g/kg body weight), alive (3 × 10 CFU/mouse), or ST + (1.5 g/kg plus 3 × 10 CFU/mouse), respectively. After 21 days of intervention, all mice except the normal mice were intragastrically administrated with diphenoxylate (10 mg/kg body weight). Defecation indexes, constipation-related intestinal factors, serum neurotransmitters, hormone levels, short-chain fatty acids (SCFAs), and intestinal microbiota were measured.
Our results showed that three interventions with ST, , and synbiotic combination (ST + . sakei) all alleviated constipation, and synbiotic intervention was superior to ST or alone in some defecation indicators. The RT-PCR and immunohistochemical experiment showed that all three interventions relieved constipation by affecting aquaporins (AQP4 and AQP8), interstitial cells of Cajal (SCF and c-Kit), glial cell-derived neurotrophic factor (GDNF), and Nitric Oxide Synthase (NOS). The three interventions exhibited a different ability to increase the serum excitatory neurotransmitters and hormones (5-hydroxytryptamine, substance P, motilin), and reduce the serum inhibitory neurotransmitters (vasoactive intestinal peptide, endothelin). The result of 16S rDNA sequencing of feces showed that synbiotic intervention significantly increased the relative abundance of beneficial bacteria such as , and regulated the gut microbes of STC mice. In conclusion, oral administration of ST or alone or combined are all effective to relieve constipation and the symbiotic use may have a promising preventive effect on STC.
慢传输型便秘（STC）是消化系统的一种常见疾病。本研究旨在评估水苏糖（ST）和副干酪乳杆菌2019（Lp2019）单独或联合使用对苯乙哌啶诱导的便秘的影响，并使用小鼠模型探讨其潜在机制。
将ICR小鼠随机分为五组。正常组和便秘模型组灌胃给予PBS。ST组、Lp2019组和合生元组分别灌胃给予ST（1.5 g/kg体重）、Lp2019（3×10⁸CFU/只小鼠）或ST+Lp2019（1.5 g/kg加3×10⁸CFU/只小鼠）。干预21天后，除正常小鼠外，所有小鼠均灌胃给予苯乙哌啶（10 mg/kg体重）。测量排便指数、便秘相关肠道因子、血清神经递质、激素水平、短链脂肪酸（SCFAs）和肠道微生物群。
我们的结果表明，ST、Lp2019和合生元组合（ST+Lp2019）的三种干预均缓解了便秘，并且在一些排便指标上，合生元干预优于单独的ST或Lp2019。RT-PCR和免疫组化实验表明，所有三种干预均通过影响水通道蛋白（AQP4和AQP8）、Cajal间质细胞（SCF和c-Kit）、胶质细胞源性神经营养因子（GDNF）和一氧化氮合酶（NOS）来缓解便秘。三种干预在增加血清兴奋性神经递质和激素（5-羟色胺、P物质、胃动素）以及降低血清抑制性神经递质（血管活性肠肽、内皮素）方面表现出不同的能力。粪便16S rDNA测序结果表明，合生元干预显著增加了双歧杆菌等有益菌的相对丰度，并调节了STC小鼠的肠道微生物群。总之，单独或联合口服ST或Lp2019均能有效缓解便秘，且联合使用可能对STC具有良好的预防作用。
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