Guo Qiang, Yao Lu, Liu Zhong, Yao Jing-Chun, Tu Peng-Fei, Zeng Ke-Wu
State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University Beijing 100191,China.
State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine,Lunan Pharmaceutical Group Co.,Ltd. Linyi 276006,China.
Zhongguo Zhong Yao Za Zhi. 2021 Feb;46(3):505-510. doi: 10.19540/j.cnki.cjcmm.20201125.403.
"Target fishing" strategy was used to investigate the direct targets and mechanism of Shouhui Tongbian Capsules on relaxing bowel. Magnetic beads cross-linked with the chemical constituents from Shouhui Tongbian Capsules were prepared. The potential target proteins were captured from the total protein lysates of rat intestine using the beads. The captured proteins were further identified by LC-MS/MS, and the associated pathways were analyzed by Cytoscape. RESULTS:: showed that 138 potential target proteins were identified, which were involved in eight signaling pathways, including tricarboxylic acid cycle, pyrimidine metabolism, sulfur metabolism, fatty acid degradation, alanine/aspartate/glutamate metabolism, arginine/proline metabolism, valine/leucine/isoleucine degradation, and β-alanine metabolism. Taken together, Shouhui Tongbian Capsules may exert relaxing bowel effect by acting on multiple signaling pathways to promote intestinal gurgling, inhibit inflammation, as well as improve intestinal barrier function, intestinal water secretion, and intestinal flora.
采用“靶向垂钓”策略研究首荟通便胶囊润肠通便的直接靶点及作用机制。制备与首荟通便胶囊化学成分交联的磁珠，利用磁珠从大鼠肠组织总蛋白裂解物中捕获潜在的靶蛋白。通过液相色谱-串联质谱法（LC-MS/MS）进一步鉴定捕获的蛋白，并利用Cytoscape软件分析相关通路。结果显示，共鉴定出138个潜在靶蛋白，涉及三羧酸循环、嘧啶代谢、硫代谢、脂肪酸降解、丙氨酸/天冬氨酸/谷氨酸代谢、精氨酸/脯氨酸代谢、缬氨酸/亮氨酸/异亮氨酸降解和β-丙氨酸代谢8条信号通路。综上所述，首荟通便胶囊可能通过作用于多条信号通路，促进肠道蠕动、抑制炎症以及改善肠道屏障功能、肠道水分泌和肠道菌群，从而发挥润肠通便作用。
