Li Xing, Chu Fu-Jiang, Jiang Shi-Lin, Jin Xiao-Bao
Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances Guangzhou 510006, China School of Life Science and Bio-pharmaceutical, Guangdong Pharmaceutical University Guangzhou 510006, China.
Zhongguo Zhong Yao Za Zhi. 2021 Jan;46(1):177-182. doi: 10.19540/j.cnki.cjcmm.20200915.403.
The aim of this paper was to investigate the effect of ethanol extract of Phellinus igniarius in lowering uric acid and changing the gut microbiome in hyperuricemia rats. A total of 36 SD rats were randomly divided into normal control group, model control group, positive drug control group, and high-dose, middle-dose and low-dose P. igniarius ethanol extract groups, with 6 rats in each group. Hyperuricemia rats were established by D-fructose combined with oteracil potassium(OAPS). One week later, the positive control group was given allopurinol 50 mg·kg(-1) intragastrically, and P. igniarius ethanol extract groups were treated with 30, 60 and 90 mg·kg(-1) drugs for 14 consecutive days. Body weight, blood glucose and serum uric acid(SUA) were monitored every week. After the model rats were administered with the ethanol extracts of P. igniarius by gavage for two weeks, the activities of creatinine, BUN, xanthine oxidase(XOD) and adenosine deaminase(ADA) were detected. The right kidney was taken to analyze the histological and morphological changes and the degree of damage to main organs of the extract of P. igniarius. The 16 S rDNA gene sequence technique was used to analyze the guts microbiota composition in feces. The results indicated that ethanol extract of P. igniarius could significantly lower the SUA level(P<0.01), while inhibiting the activities of XOD and ADA(P<0.05, P<0.01). Histological examination showed that the allopurine group showed slight renal tubular dilation and inflammatory cell infiltration compared with the normal group, with no significant difference between the P. igniarius ethanol extract groups and the normal group. The 16 S sequencing results showed that the composition of gut microbiota has changed in each group. Therefore, ethanol extracts of P. igniarius may reduce the level of SUA in rats by inhibiting the activities of XOD and ADA, with a certain effect on the composition of gut microbiota.
本文旨在研究桑黄乙醇提取物对高尿酸血症大鼠降低尿酸及改变肠道微生物群的影响。将36只SD大鼠随机分为正常对照组、模型对照组、阳性药物对照组、桑黄乙醇提取物高剂量组、中剂量组和低剂量组，每组6只。采用D-果糖联合氧嗪酸钾（OAPS）建立高尿酸血症大鼠模型。1周后，阳性对照组给予别嘌醇50 mg·kg⁻¹灌胃，桑黄乙醇提取物组分别给予30、60和90 mg·kg⁻¹药物连续灌胃14天。每周监测体重、血糖和血清尿酸（SUA）。模型大鼠经口灌胃桑黄乙醇提取物两周后，检测肌酐、尿素氮、黄嘌呤氧化酶（XOD）和腺苷脱氨酶（ADA）的活性。取右肾分析桑黄提取物对主要器官的组织学和形态学变化及损伤程度。采用16S rDNA基因序列技术分析粪便中肠道微生物群的组成。结果表明，桑黄乙醇提取物能显著降低SUA水平（P<0.01），同时抑制XOD和ADA的活性（P<0.05，P<0.01）。组织学检查显示，与正常组相比，别嘌醇组肾小管有轻度扩张和炎性细胞浸润，桑黄乙醇提取物组与正常组之间无显著差异。16S测序结果表明，各组肠道微生物群的组成均发生了变化。因此，桑黄乙醇提取物可能通过抑制XOD和ADA的活性降低大鼠SUA水平，对肠道微生物群的组成有一定影响。
