Korthuis R J, Grisham M B, Granger D N
Department of Biomedical Sciences, College of Veterinary Medicine, University of Missouri-Columbia 65211.
Am J Physiol. 1988 May;254(5 Pt 2):H823-7. doi: 10.1152/ajpheart.1988.254.5.H823.
To determine whether leukocytes play an important role in the pathogenesis of the vascular injury (increased vascular permeability and resistance) associated with ischemia-reperfusion, isolated canine gracilis muscles were perfused with autologous whole blood or with whole blood that had been depleted of leukocytes (primarily granulocytes) using Leukopak filters. The osmotic reflection coefficient for total plasma proteins, isogravimetric capillary pressure, and total vascular resistance was determined for the following conditions: control, ischemia (4 h inflow occlusion) plus reperfusion with whole blood, and ischemia plus reperfusion with granulocyte-depleted whole blood. Reperfusion with whole blood was associated with a reduction in the osmotic reflection coefficient from 0.96 to 0.61, whereas isogravimetric capillary pressure was reduced by 40%, indicating a dramatic increase in vascular permeability. Total vascular resistance was increased approximately twofold. Reperfusion with leukocyte-depleted blood largely prevented the increases in vascular permeability and resistance. These data suggest that leukocytes play a major role in the pathogenesis of ischemia-reperfusion injury in skeletal muscle.
为了确定白细胞在与缺血再灌注相关的血管损伤（血管通透性增加和阻力增加）发病机制中是否起重要作用，使用白细胞过滤器对分离的犬股薄肌灌注自体全血或已去除白细胞（主要是粒细胞）的全血。在以下条件下测定总血浆蛋白的渗透反射系数、等重量毛细血管压力和总血管阻力：对照、缺血（4小时入流阻塞）加全血再灌注，以及缺血加去粒细胞全血再灌注。全血再灌注与渗透反射系数从0.96降至0.61相关，而异重量毛细血管压力降低了40%，表明血管通透性显著增加。总血管阻力增加了约两倍。用去白细胞血液再灌注在很大程度上防止了血管通透性和阻力的增加。这些数据表明白细胞在骨骼肌缺血再灌注损伤的发病机制中起主要作用。
