Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, Bahia, Brazil.
Neotrop Entomol. 2021 Oct;50(5):846-849. doi: 10.1007/s13744-021-00853-7. Epub 2021 Mar 1.
Trigona spinipes Fabricius is a stingless bee with wide geographical distribution. Although being sometimes considered an agricultural pest, in fact, it has great pollinating potential, and therefore economic interest. Conventional and molecular cytogenetic techniques have been little used to verify genetic diversity in this species, despite its potential to reveal information about the reorganization of the genome having been demonstrated in other species. Conventional cytogenetic techniques, fluorochrome staining, and fluorescent in situ hybridization with 18S rDNA, telomeric, and microsatellite probes (GA) were used in this study to characterize and compare T. spinipes from different locations. The karyotypes showed a conserved chromosome number 2n = 34; however, geographic variations were verified in the different features and cytogenetic techniques analyzed, such as karyotype formulas, fluorocrome staining, and FISH. Although the 18S rDNA probe revealed the same number of markings in five rDNA clusters, the chromosomal pairs containing these markers varied between studied locations. The probe for microsatellite (GA) also showed polymorphisms within this species. The results reveal that T. spinipes has many intraspecific differences, revealing a higher chromosomal variation than expected.
黄猄蚁是一种分布广泛的无刺蜂。尽管有时被认为是农业害虫，但实际上它具有很大的授粉潜力，因此具有经济意义。尽管已经证明其他物种的基因组重排信息可以通过常规和分子细胞遗传学技术来验证，但这些技术在该物种中的遗传多样性验证中很少使用。本研究采用常规细胞遗传学技术、荧光染料染色和 18S rDNA、端粒和微卫星探针（GA）的荧光原位杂交技术，对来自不同地点的黄猄蚁进行了特征描述和比较。核型显示出保守的染色体数目 2n = 34；然而，在不同的特征和分析的细胞遗传学技术中，如核型公式、荧光染料染色和 FISH，都证实了地理变异。尽管 18S rDNA 探针在五个 rDNA 簇中显示出相同数量的标记，但含有这些标记的染色体对在不同的研究地点之间存在差异。微卫星（GA）探针也显示出该物种内的多态性。结果表明，黄猄蚁具有许多种内差异，显示出比预期更高的染色体变异。
