从 sp. PF1464 中提取的一种抗真菌真菌色素—— Amphiol。

Amphiol, an Antifungal Fungal Pigment from sp. PF1464.

机构信息

Department of System Chemotherapy and Molecular Sciences, Division of Bioinformatics and Chemical Genomics, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501, Japan.

Department of Biotechnology, The University of Tokyo, Tokyo 113-8657, Japan.

出版信息

J Nat Prod. 2021 Apr 23;84(4):986-992. doi: 10.1021/acs.jnatprod.0c01010. Epub 2021 Mar 1.

DOI:10.1021/acs.jnatprod.0c01010

PMID:33646775

Abstract

An antifungal metabolite, designated amphiol (), was isolated from the culture broth of the fungus sp. PF1464. It exists as a mixture of inseparable tautomers, an acetal form and a keto form. The chemical structure was determined by spectroscopic analyses and chemical derivatization. Amphiol () showed antifungal but not antibacterial activities, while yeast mutant cells lacking ergosterol biosynthetic genes were less sensitive, implying a fungal specific, membrane-related mechanism of action.

摘要

从真菌 sp. PF1464 的发酵液中分离得到一种抗真菌代谢产物，命名为 amphio l ()。它以不可分离的互变异构体、缩醛形式和酮式形式存在。通过光谱分析和化学衍生化确定了其化学结构。Amphiol () 表现出抗真菌活性，但没有抗菌活性，而缺乏麦角固醇生物合成基因的酵母突变细胞则不那么敏感，这表明它具有真菌特异性、与膜相关的作用机制。