Tang Yue, Hu Yang, Zhou Pei, Wang Chunxiao, Tao Han, Wu Yuangen
School of Liquor and Food Engineering; Guizhou Province Key Laboratory of Agriculture Engineering and Biology; Key Laboratory of Plant Resource Conservation and German Innovation in Mountain Region (Ministry of Education), Guizhou University, Huaxi District, Guiyang 550025, China.
Key Laboratory of Urban Agriculture Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
J Agric Food Chem. 2021 Mar 10;69(9):2884-2893. doi: 10.1021/acs.jafc.1c00925. Epub 2021 Mar 1.
Although the colorimetric methods can easily meet the demands of point-of-care and ease-of-use for antibiotic detection, they still face many challenges in the accuracy and stability of assay. Herein, a facile and stable colorimetric aptasensor is first developed for kanamycin residue detection based on the aptamer-enhanced peroxidase-mimicking activity of layered WS nanosheets. The investigation confirmed that aptamer sequences can improve the affinity of nanosheets to the chromogenic substrate 3,3'',5,5''-tetramethylbenzidine, resulting in a significant increase of the peroxidase-mimicking activity. Under the optimal conditions, the limit of detection of the proposed colorimetric aptasensor for kanamycin was determined to be as low as 0.6 μM, and such an aptasensor displays excellent selectivity against other competitive antibiotics. Moreover, further studies have verified the applicability of the established colorimetric aptasensor in several actual samples, indicating that the aptasensor may have bright application prospects for kanamycin detection in livestock husbandry and agriculture samples.
尽管比色法能够轻松满足即时检测和抗生素检测易用性的要求，但它们在检测的准确性和稳定性方面仍面临诸多挑战。在此，基于适配体增强的层状WS纳米片的过氧化物酶模拟活性，首次开发了一种用于卡那霉素残留检测的简便且稳定的比色适配体传感器。研究证实，适配体序列可提高纳米片对显色底物3,3'',5,5''-四甲基联苯胺的亲和力，从而显著提高过氧化物酶模拟活性。在最佳条件下，所提出的卡那霉素比色适配体传感器的检测限低至0.6 μM，并且这种适配体传感器对其他竞争性抗生素具有出色的选择性。此外，进一步研究验证了所建立的比色适配体传感器在几种实际样品中的适用性，表明该适配体传感器在畜牧业和农业样品中卡那霉素检测方面可能具有广阔的应用前景。
