Gernert K M, Smith R, Carter D C
NASA/Marshall Space Flight Center, Huntsville, Alabama 35812.
Anal Biochem. 1988 Jan;168(1):141-7. doi: 10.1016/0003-2697(88)90021-8.
A simple device is described for controlling vapor equilibrium in macromolecular crystallization as applied to the protein crystal growth technique commonly referred to as the "hanging drop" method. Crystal growth experiments with hen egg white lysozyme have demonstrated control of the nucleation rate. Nucleation rate and final crystal size have been found to be highly dependent upon the rate at which critical supersaturation is approached. Slower approaches show a marked decrease in the nucleation rate and an increase in crystal size.
本文描述了一种简单装置，用于控制大分子结晶中的蒸汽平衡，该装置应用于通常被称为“悬滴”法的蛋白质晶体生长技术。用鸡蛋清溶菌酶进行的晶体生长实验证明了对成核速率的控制。已发现成核速率和最终晶体尺寸高度依赖于接近临界过饱和度的速率。较慢的接近速率显示成核速率显著降低，晶体尺寸增加。
