Hu Y T, Yu F, Tang X Y, Wu W Z, Zhang P, Hu Z H, Chen J H, Xing X D, Xiao Y H
Department of Stomatology, 920 Hospital of Joint Logistics Support Force, PLA, Kunming 650032, China; Department of Stomatology, The First Hospital of Kunming, Kunming 650011, China.
Department of Stomatology, 920 Hospital of Joint Logistics Support Force, PLA, Kunming 650032, China.
J Mech Behav Biomed Mater. 2021 May;117:104414. doi: 10.1016/j.jmbbm.2021.104414. Epub 2021 Feb 23.
The application of pit and fissure sealants is a well-established method to prevent and treat early childhood caries. Resin-based sealants with antibacterial properties provide additional benefits for caries prevention in a cariogenic oral environment. The objective of this study was to evaluate the effect of an antibacterial core-shell AgBr/cationic polymer nanocomposite (AgBr/BHPVP) on the properties of a resin-based pit and fissure sealant. A commercialized pit and fissure sealant without fluoride, Concise (3M, ESPE, USA), was used as the parent material and negative control. Experimental antibacterial sealants were formulated by the addition of AgBr/BHPVP nanoparticles at mass fractions of 0.5, 1.0, and 1.5 wt% to the parent material. A fluoride-releasing sealant, Clinpro (3M, ESPE), was used as the positive control. Bacterial colony-forming unit (CFU) counts, metabolic activity tests, field emission-scanning electron microscopy (FE-SEM), and confocal laser scanning microscopy (CLSM) observations were used to evaluate the antibacterial properties of AgBr/BHPVP-modified sealants against Streptococcus mutans before and after five months of aging. The Vickers microhardness, degree of conversion, and microleakage level of the sealants were also investigated. According to the results of CFU counts and metabolic tests, sealants containing AgBr/BHPVP showed better contact-killing bactericidal activity against S. mutans than the two commercial sealants, irrespective of aging conditions (both P < 0.05). The AgBr/BHPVP-modified sealants also showed a significant inhibitory effect on the planktonic S. mutans around the cured sealant surfaces. In addition, the Vickers microhardness, degree of conversion, and microleakage level of the parent material were not damaged by modification with AgBr/BHPVP (P > 0.05). AgBr/BHPVP-modified pit and fissure sealant with a dual bactericidal mechanism is a promising option for the prevention of pit and fissure caries.
窝沟封闭剂的应用是预防和治疗幼儿龋齿的一种成熟方法。具有抗菌性能的树脂基封闭剂在致龋性口腔环境中为预防龋齿提供了额外的益处。本研究的目的是评估抗菌核壳AgBr/阳离子聚合物纳米复合材料（AgBr/BHPVP）对树脂基窝沟封闭剂性能的影响。一种不含氟的商业化窝沟封闭剂Concise（美国3M公司，ESPE）用作母体材料和阴性对照。通过向母体材料中添加质量分数为0.5%、1.0%和1.5%的AgBr/BHPVP纳米颗粒来配制实验性抗菌封闭剂。一种含氟释放封闭剂Clinpro（美国3M公司，ESPE）用作阳性对照。采用细菌菌落形成单位（CFU）计数、代谢活性测试、场发射扫描电子显微镜（FE-SEM）和共聚焦激光扫描显微镜（CLSM）观察来评估AgBr/BHPVP改性封闭剂在老化五个月前后对变形链球菌的抗菌性能。还研究了封闭剂的维氏显微硬度、转化率和微渗漏水平。根据CFU计数和代谢测试结果，无论老化条件如何，含AgBr/BHPVP的封闭剂对变形链球菌的接触杀灭杀菌活性均优于两种商业化封闭剂（均P<0.05）。AgBr/BHPVP改性封闭剂对固化封闭剂表面周围的浮游变形链球菌也有显著的抑制作用。此外，母体材料的维氏显微硬度、转化率和微渗漏水平未因AgBr/BHPVP改性而受损（P>0.05）。具有双重杀菌机制的AgBr/BHPVP改性窝沟封闭剂是预防窝沟龋的一个有前景的选择。
