Department of Head and Neck Surgery, Kanazawa Medical University, Ishikawa, Japan; Department of Pathology and Laboratory Medicine, Kanazawa Medical University, Ishikawa, Japan.
Department of Pathology and Laboratory Medicine, Kanazawa Medical University, Ishikawa, Japan.
Pathol Res Pract. 2021 Apr;220:153386. doi: 10.1016/j.prp.2021.153386. Epub 2021 Feb 17.
Warthin tumour (WT) is a benign tumour of the salivary gland that proliferates in both glandular epithelial and lymphoid tissue components, and rarely exhibits cystic changes. T follicular helper cells (Tfh) are involved in the formation and maintenance of germinal centres, the differentiation of B cells into plasma cells, and the maintenance of helper T cell type 2 (Th2)-dominant humoral immune responses. T-bet induces differentiation into helper T cell type 1 (Th1) by suppressing differentiation into Tfh and enhances cellular immune responses. The objective of this study was to enhance our understanding of the immune responses and relationship between Tfh and Th1 cells in patients with WTs. In this study, we classified WTs (n = 64) into solid-type (n = 25) and cyst-type (n = 39). We also performed immunostaining of the Tfh markers CXCR5 and CD40 L, and the Th1 marker T-bet for statistical analysis. The cyst-type exhibited significant atrophy of the germinal centre area (P = 0.0019), significantly fewer Tfh-positive lymphocytes in germinal centres (P < 0.0001), and significantly more T-bet-positive lymphocytes in the epithelium (P = 0.0017). We observed that Tfh were involved in the formation and maintenance of lymphoid follicles in WTs. In the cyst-type, Th2-dominant humoral immune responses were suppressed, and Th1-dominant cellular immune responses may have caused damage to tumour tissue.
沃辛瘤（WT）是一种良性涎腺肿瘤，在腺体上皮和淋巴组织成分中均有增殖，很少出现囊性变。滤泡辅助性 T 细胞（Tfh）参与生发中心的形成和维持、B 细胞向浆细胞的分化以及辅助性 T 细胞 2（Th2）型优势体液免疫反应的维持。T 细胞转录因子（T-bet）通过抑制向 Tfh 分化来诱导向辅助性 T 细胞 1（Th1）分化，并增强细胞免疫反应。本研究旨在增强我们对 WT 患者中 Tfh 和 Th1 细胞免疫反应的理解。在这项研究中，我们将 WT（n=64）分为实体型（n=25）和囊性型（n=39）。我们还对 Tfh 标志物 CXCR5 和 CD40L 以及 Th1 标志物 T-bet 进行了免疫染色，并进行了统计学分析。囊性型的生发中心区域显著萎缩（P=0.0019），生发中心内 Tfh 阳性淋巴细胞明显减少（P<0.0001），上皮内 T-bet 阳性淋巴细胞明显增多（P=0.0017）。我们观察到 Tfh 参与了 WT 中淋巴滤泡的形成和维持。在囊性型中，Th2 型优势体液免疫反应受到抑制，而 Th1 型优势细胞免疫反应可能导致肿瘤组织损伤。
