Team of Applied Microbiology and Biotechnologies, BioMare Laboratory, Faculty of Sciences, Chouaib Doukkali University, El Jadida 24000, Morocco.
Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain.
Food Res Int. 2021 Feb;140:109958. doi: 10.1016/j.foodres.2020.109958. Epub 2020 Dec 8.
Green tea infusion is one of the most widely drunk beverages worldwide due to its health benefits associated with microelements, essential oils, and polyphenols, etc. Several studies have reported that green tea is subjected to contamination by various toxigenic fungi. Thus, this work aims to investigate the co-occurrence of 15 mycotoxins [four aflatoxins (AFB1, AFB2, AFG1, AFG2), ochratoxin A (OTA), beauvericin (BEA), four enniatins (ENA, ENA1, ENB, ENB1), zearalenone (ZEN), alternariol (AOH), tentoxin (TENT), T-2 and HT-2 toxins] in green tea samples available in Morocco by liquid chromatography tandem mass spectrometry method. Analytical and consumption data were then used to assess the dietary exposure for the population. Out of 111 total green tea samples, 62 (56%) were contaminated by at least one mycotoxin. The most found mycotoxins in samples were AOH (40%), ZEN (35%), AFG1 (2%), AFB2 (2%), ENB (2%) and TENT (1%). The highest level was found for ZEN with 45.8 ng/g. There is no sample that exceeded the recommended levels set by European Pharmacopoeia for certain mycotoxins in plant material. Although multi-mycotoxin co-occurred in samples (33%), the probable estimated daily intake values show that the intake of mycotoxins through the consumption of green tea does not represent a risk for the population.
绿茶是世界上饮用最广泛的饮料之一，因为它含有微量元素、精油和多酚等有益健康的成分。有几项研究报告称，绿茶受到各种产毒真菌的污染。因此，本工作旨在通过液相色谱串联质谱法调查摩洛哥市售绿茶样品中 15 种霉菌毒素（四种黄曲霉毒素（AFB1、AFB2、AFG1、AFG2）、赭曲霉毒素 A（OTA）、 beauvericin（BEA）、四种 enniatins（ENA、ENA1、ENB、ENB1）、玉米赤霉烯酮（ZEN）、alternariol（AOH）、 tentoxin（TENT）、T-2 和 HT-2 毒素）的共同污染情况。然后，利用分析和消费数据评估人群的饮食暴露情况。在 111 份绿茶样品中，有 62 份（56%）至少受到一种霉菌毒素的污染。样品中发现的主要霉菌毒素为 AOH（40%）、ZEN（35%）、AFG1（2%）、AFB2（2%）、ENB（2%）和 TENT（1%）。最高含量为 ZEN，为 45.8 ng/g。没有一个样品超过欧洲药典对植物材料中某些霉菌毒素规定的推荐水平。尽管样品中存在多种霉菌毒素的共同污染（33%），但可能的每日估计摄入量值表明，通过饮用绿茶摄入霉菌毒素不会对人群构成风险。
