College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road, Haidian District, Beijing 100083, PR China; Beijing Higher Institution Engineering Research Center of Animal Product, China Agricultural University, No. 17 Qinghua East Road, Haidian District, Beijing 100083, PR China.
Department of Biotechnology, Universidad Tecnológica Metropolitana, Las Palmeras 3360, Ñuñoa, Santiago, Chile.
Food Res Int. 2021 Feb;140:109989. doi: 10.1016/j.foodres.2020.109989. Epub 2020 Dec 24.
In this study, the high-throughput Illumina HiSeq 2000 mRNA sequencing technique was used to investigate the transcriptome response of Escherichia coli O157:H7 exposed to ohmic heating (OH) and water bath heating (WB). Compared to untreated samples, a total of 293, 516, and 498 genes showed differential expression after HVOH (high voltage short time ohmic heating), LVOH (low voltage long time ohmic heating), and WB, respectively. Therefore, LVOH had the potential to cause comparable effects on the transcriptome of E. coli O157:H7 as compared to WB, but not HVOH. These results indicated that additional non-thermal effects were not reflected on transcriptome of E. coli O157:H7 using both HVOH and LVOH, in particular the HVOH. Most of differentially expressed genes involved in information storage and processing, and cellular processes and signaling showed up-regulation whereas most of genes related to the metabolism were down-regulated after HVOH, LVOH, and WB. In addition, more attention needs to be paid to the up-regulation of a large number of virulence genes, which might increase the ability of surviving E. coli O157:H7 to infect host cells after HVOH, LVOH, and WB. This transcriptomic study on the response of E. coli O157:H7 to OH protomes the understanding of inactivation mechanism of OH on the molecular level and opens the door to future studies for OH.
在这项研究中，使用高通量 Illumina HiSeq 2000 mRNA 测序技术来研究大肠杆菌 O157:H7 暴露于欧姆加热 (OH) 和水浴加热 (WB) 时的转录组反应。与未处理的样品相比，HVOH（高压短时间欧姆加热）、LVOH（低压长时间欧姆加热）和 WB 处理后，总共有 293516 个和 498 个基因表现出差异表达。因此，LVOH 对大肠杆菌 O157:H7 的转录组可能产生与 WB 相当的影响，但 HVOH 则不然。这些结果表明，使用 HVOH 和 LVOH 都没有在大肠杆菌 O157:H7 的转录组上反映出额外的非热效应，特别是 HVOH。大多数差异表达的基因涉及信息存储和处理、细胞过程和信号转导，表现出上调，而大多数与代谢相关的基因在 HVOH、LVOH 和 WB 后下调。此外，需要更多关注大量毒力基因的上调，这可能会增加 HVOH、LVOH 和 WB 后大肠杆菌 O157:H7 感染宿主细胞的存活能力。这项关于 OH 对大肠杆菌 O157:H7 蛋白质组反应的转录组研究增进了我们对 OH 在分子水平上对细菌失活动力学的理解，并为 OH 的未来研究开辟了道路。
