School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, People's Republic of China.
School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, People's Republic of China.
Food Res Int. 2021 Feb;140:110011. doi: 10.1016/j.foodres.2020.110011. Epub 2020 Dec 15.
The ovalbumin (OVA)-pectin (PEC)-sodium alginate (SA)-Vitamin D (VD) complex nanoparticles were fabricated by antisolvent precipitation method, and the excellent encapsulation efficiency and loading capacity of VD were obtained by 96.6% and 2.8%, respectively. Compared with ternary OVA-PEC-VD complexes, the addition of SA with strong negative charge effectively regulated the OVA-PEC complexes and significantly improved the stability of OVA-PEC-SA-VD complex nanoparticles with preferable size as small as 126 nm. The storage stability was also investigated after low temperature storage for 31 d, and the particle size of quaternary complexes was increased only 40 nm. In vitro digestion results elucidated that the complex nanoparticles had good stability in the simulated gastric fluid, and almost completely released in the simulated intestinal fluid confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) experiments and scanning electron microscope (SEM) images. The release kinetics study clarified that it was close to Fick release. Fluorescence and Fourier transform infrared spectroscopy (FTIR) experiments showed that quaternary complex nanoparticles were mainly combined by electrostatic, hydrophobic and hydrogen bonding interactions. The novel quaternary protein-polysaccharide complexes have excellent stability and great sustained-release performance for VD, which may be helpful for the digestion and absorption of vitamin by human body, thus have potential applications in the food and drug industry.
卵清蛋白（OVA）-果胶（PEC）-海藻酸钠（SA）-维生素 D（VD）复合纳米粒子是通过反溶剂沉淀法制备的，VD 的包封效率和载药量分别达到了 96.6%和 2.8%。与三元 OVA-PEC-VD 复合物相比，添加带强负电荷的 SA 有效调节了 OVA-PEC 复合物，并显著提高了 OVA-PEC-SA-VD 复合纳米粒子的稳定性，其粒径小至 126nm。还研究了低温储存 31d 后的储存稳定性，发现四元复合物的粒径仅增加了 40nm。体外消化结果表明，复合纳米粒子在模拟胃液中具有良好的稳定性，并且通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）实验和扫描电子显微镜（SEM）图像证实几乎完全在模拟肠液中释放。释放动力学研究表明其接近菲克释放。荧光和傅里叶变换红外光谱（FTIR）实验表明，四元复合纳米粒子主要通过静电、疏水和氢键相互作用结合。新型的蛋白质-多糖四元复合物具有优异的稳定性和 VD 的持续释放性能，这可能有助于人体对维生素的消化和吸收，因此在食品和药物工业中有潜在的应用。
