Ramirez-Priego Patricia, Estévez M-Carmen, Díaz-Luisravelo Heriberto J, Manclús Juan J, Montoya Ángel, Lechuga Laura M
Nanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, BIST and CIBER-BBN, Campus UAB, Bellaterra, 08193, Barcelona, Spain.
Nanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, BIST and CIBER-BBN, Campus UAB, Bellaterra, 08193, Barcelona, Spain.
Anal Chim Acta. 2021 Apr 1;1152:338276. doi: 10.1016/j.aca.2021.338276. Epub 2021 Feb 3.
Due to the large quantities of pesticides extensively used and their impact on the environment and human health, a prompt and reliable sensing technique could constitute an excellent tool for in-situ monitoring. With this aim, we have applied a highly sensitive photonic biosensor based on a bimodal waveguide interferometer (BiMW) for the rapid, label-free, and specific quantification of fenitrothion (FN) directly in tap water samples. After an optimization protocol, the biosensor achieved a limit of detection (LOD) of 0.29 ng mL (1.05 nM) and a half-maximal inhibitory concentration (IC) of 1.71 ng mL (6.09 nM) using a competitive immunoassay and employing diluted tap water. Moreover, the biosensor was successfully employed to determine FN concentration in blind tap water samples obtaining excellent recovery percentages with a time-to-result of only 20 min without any sample pre-treatment. The features of the biosensor suggest its potential application for real time, fast and sensitive screening of FN in water samples as an analytical tool for the monitoring of the water quality.
由于大量农药被广泛使用及其对环境和人类健康的影响，一种快速可靠的传感技术可成为现场监测的优良工具。为此，我们应用了一种基于双峰波导干涉仪（BiMW）的高灵敏度光子生物传感器，用于直接对自来水样品中的杀螟硫磷（FN）进行快速、无标记和特异性定量。经过优化方案后，该生物传感器采用竞争性免疫测定法并使用稀释的自来水，实现了0.29 ng/mL（1.05 nM）的检测限（LOD）和1.71 ng/mL（6.09 nM）的半最大抑制浓度（IC）。此外，该生物传感器成功用于测定未知自来水样品中的FN浓度，获得了优异的回收率，且仅需20分钟即可得出结果，无需任何样品预处理。该生物传感器的特性表明其作为水质监测分析工具，在水样中FN的实时、快速和灵敏筛查方面具有潜在应用价值。
