Tecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey 64849, Mexico.
Departamento de Física y Matemáticas, Universidad Iberoamericana, Prolongación Paseo de la Reforma 880, Mexico City 01219, Mexico.
Biosensors (Basel). 2023 Mar 10;13(3):370. doi: 10.3390/bios13030370.
The correct detection and quantification of pollutants in water is key to regulating their presence in the environment. Biosensors offer several advantages, such as minimal sample preparation, short measurement times, high specificity and sensibility and low detection limits. The purpose of this review is to explore the different types of optical biosensors, focusing on their biological elements and their principle of operation, as well as recent applications in the detection of pollutants in water. According to our literature review, 33% of the publications used fluorescence-based biosensors, followed by surface plasmon resonance (SPR) with 28%. So far, SPR biosensors have achieved the best results in terms of detection limits. Although less common (22%), interferometers and resonators (4%) are also highly promising due to the low detection limits that can be reached using these techniques. In terms of biological recognition elements, 43% of the published works focused on antibodies due to their high affinity and stability, although they could be replaced with molecularly imprinted polymers. This review offers a unique compilation of the most recent work in the specific area of optical biosensing for water monitoring, focusing on both the biological element and the transducer used, as well as the type of target contaminant. Recent technological advances are discussed.
正确检测和定量水中的污染物对于调节其在环境中的存在至关重要。生物传感器具有几个优点,例如最小的样品制备、短的测量时间、高特异性和灵敏度以及低检测限。本文旨在探讨不同类型的光学生物传感器,重点介绍它们的生物元件及其工作原理,以及在水中污染物检测中的最新应用。根据我们的文献综述,33%的出版物使用基于荧光的生物传感器,其次是表面等离子体共振(SPR),占 28%。到目前为止,SPR 生物传感器在检测限方面取得了最佳效果。尽管(22%)不太常见,但干涉仪和谐振器(4%)也极具前景,因为使用这些技术可以达到低检测限。在生物识别元件方面,由于其高亲和力和稳定性,43%的已发表工作集中在抗体上,尽管它们可以被分子印迹聚合物取代。本文对用于水质监测的光学生物传感特定领域的最新工作进行了独特的汇编,重点介绍了生物元件和所用换能器以及目标污染物的类型。讨论了最近的技术进展。