双歧杆菌染色体和质粒 DNA 的分离。

Isolation of Chromosomal and Plasmid DNA from Bifidobacteria.

机构信息

School of Microbiology and APC Microbiome Ireland, University College Cork, Cork, Ireland.

Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Villaviciosa, Spain.

出版信息

Methods Mol Biol. 2021;2278:21-29. doi: 10.1007/978-1-0716-1274-3_3.

DOI:10.1007/978-1-0716-1274-3_3

PMID:33649945

Abstract

Rapid and efficient protocols aimed at the isolation and purification of DNA for the purpose of downstream applications, such as cloning, PCR, Southern blotting, or sequencing, are essential for genetic, biochemical, and molecular biological analyses of a given bacterium. The protocols herein presented provide a robust and efficient method for the isolation of chromosomal and plasmid DNA from Bifidobacterium strains by organic extraction. The methods are simple, and the yield, purity, and quality of the DNA are adequate to perform various downstream applications including next-generation sequencing.

摘要

快速有效的 DNA 分离和纯化方案对于遗传、生化和分子生物学分析给定细菌至关重要，这些方案旨在为下游应用（如克隆、PCR、Southern 印迹或测序）提供目的 DNA。本文提出的方法通过有机抽提从双歧杆菌菌株中分离染色体和质粒 DNA，提供了一种强大而有效的方法。这些方法简单，所获得的 DNA 的产量、纯度和质量足以进行各种下游应用，包括下一代测序。