Department of Biomedical Sciences, Section of Physiology, University of Cagliari, Monserrato, CA, Italy.
Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
Geroscience. 2021 Aug;43(4):1783-1798. doi: 10.1007/s11357-021-00342-0. Epub 2021 Mar 1.
To evaluate whether a peculiar plasma profile of fatty acids and endocannabinoidome (eCBome)-related mediators may be associated to longevity, we assessed them in octogenarians (Old; n=42) living in the east-central mountain area of Sardinia, a High-Longevity Zone (HLZ), compared to sexagenarian (Young; n=21) subjects from the same area, and to Olds (n=22) from the Northern Sardinia indicated as Lower-Longevity Zone (LLZ). We found significant increases in conjugated linoleic acid (CLA) and heptadecanoic acid (17:0) levels in Old-HLZ with respect to younger subjects and Old-LLZ subjects. Young-HLZ subjects exhibited higher circulating levels of pentadecanoic acid (15:0) and retinol. Palmitoleic acid (POA) was elevated in both Young and Old subjects from the HLZ. eCBome profile showed a significantly increased plasma level of the two endocannabinoids, N-arachidonoyl-ethanolamine (AEA) and 2-arachidonoyl-glycerol (2-AG) in Old-HLZ subjects compared to Young-HLZ and Old-LLZ respectively. In addition, we found increased N-oleoyl-ethanolamine (OEA), 2-linoleoyl-glycerol (2-LG) and 2-oleoyl-glycerol (2-OG) levels in Old-HLZ group with respect to Young-HLZ (as for OEA an d 2-LG) and both the Old-LLZ and Young-HLZ for 2-OG. The endogenous metabolite of docosahexaenoic acid (DHA), N-docosahexaenoyl-ethanolamine (DHEA) was significantly increased in Old-HLZ subjects. In conclusion, our results suggest that in the HLZ area, Young and Old subjects exhibited a favourable, albeit distinctive, fatty acids and eCBome profile that may be indicative of a metabolic pattern potentially protective from adverse chronic conditions. These factors could point to a suitable physiological metabolic pattern that may counteract the adverse stimuli leading to age-related disorders such as neurodegenerative and metabolic diseases.
为了评估特殊的脂肪酸和内源性大麻素（eCBome）相关介质的血浆谱是否与长寿有关，我们评估了居住在撒丁岛中东部山区（高长寿区，HLZ）的 80 岁老人（Old；n=42）、同地区的 60 岁老人（Young；n=21）以及来自撒丁岛北部的被称为低长寿区（LLZ）的 80 岁老人（Old；n=22）。我们发现与年轻组和 LLZ 组相比，HLZ 组 Old 组的共轭亚油酸（CLA）和十七烷酸（17:0）水平显著升高。HLZ 组的 Young 组表现出更高的十五烷酸（15:0）和视黄醇循环水平。棕榈油酸（POA）在 HLZ 的年轻和老年组中均升高。在 HLZ 的年轻和老年组中，内源性大麻素谱显示两种内源性大麻素，N-花生四烯酰乙醇胺（AEA）和 2-花生四烯酰甘油（2-AG）的血浆水平显著升高。此外，我们发现与 Young-HLZ 相比，Old-HLZ 组的 N-油酰乙醇胺（OEA）、2-亚油酰甘油（2-LG）和 2-油酰甘油（2-OG）水平升高，与 Young-HLZ 相比（对于 OEA 和 2-LG）以及与 Old-LLZ 和 Young-HLZ 相比，2-OG 水平升高。二十二碳六烯酸（DHA）的内源性代谢物，N-二十二碳六烯酰乙醇胺（DHEA）在 Old-HLZ 组中显著增加。总之，我们的结果表明，在 HLZ 地区，年轻和老年组表现出有利的、尽管不同的脂肪酸和内源性大麻素谱，这可能表明潜在的保护性代谢模式，可预防不利的慢性疾病。这些因素可能指向一种合适的生理代谢模式，可能抵消导致与年龄相关的疾病（如神经退行性和代谢疾病）的不利刺激。
