Marine Research Division, AZTI, Basque Research and Technology Alliance (BRTA), Sukarrieta, Spain.
Microbiology Unit, Department of Biology, Healthcare and the Environment, Faculty of Pharmacy and Food Science, University of Barcelona, Barcelona, Spain.
Environ Microbiol. 2021 Sep;23(9):5030-5041. doi: 10.1111/1462-2920.15436. Epub 2021 Mar 2.
Pseudomonas aeruginosa PAO1 membrane vesicles (MVs) are known to play a role in cell-to-cell communication. Several studies have shown that the MV composition and physicochemical properties vary according to the bacterial growth stage, but the impact this might have on the externalization of RNA via MVs has not been addressed. Therefore, a study to characterize the RNA content from MVs retrieved at different growth phases was conducted. First, the transcriptome analyses revealed a higher abundance of around 300 RNA species in MVs when compared with the cells. The vesiculation rate along the growth curve was determined, showing that the release of MVs increased during the transition to the stationary phase, whereas it decreased in the late stationary phase. RNA-seq of MVs retrieved along the transition to the stationary phase demonstrated that the RNA cargo of vesicles did not vary. However, the amount of smaller RNAs (<200 nt) inside MVs retrieved in the late exponential phase was higher than in the stationary phase MVs. These results indicate that the externalization of RNA via MVs occurs during late exponential phase and implies the secretion of different types of MVs during growth.
铜绿假单胞菌 PAO1 膜泡 (MVs) 已知在细胞间通讯中发挥作用。多项研究表明，MV 的组成和理化性质根据细菌的生长阶段而变化，但这对通过 MV 外化 RNA 的影响尚未得到解决。因此，进行了一项研究，以表征从不同生长阶段回收的 MV 中的 RNA 含量。首先，转录组分析显示，与细胞相比，MV 中的大约 300 种 RNA 丰度更高。确定了沿生长曲线的囊泡化率，结果表明，在向静止期过渡时 MV 的释放增加，而在静止后期则减少。在过渡到静止期过程中回收的 MV 的 RNA-seq 表明，囊泡的 RNA 货物没有变化。然而，在指数后期回收的 MV 内的较小 RNA（<200nt）的量高于在静止期 MV 内的量。这些结果表明，RNA 通过 MV 的外化发生在指数后期，并暗示在生长过程中分泌了不同类型的 MV。
