Department of Ultrasonic Imaging, First Affiliated Hospital, Wenzhou Medical University, China.
Adv Clin Exp Med. 2021 Feb;30(2):147-152. doi: 10.17219/acem/130607.
Resveratrol (RES) is a polyphenolic compound and natural phytoalexin that plays a potential role in various human diseases. Studies have confirmed that RES has an important function in cardioprotection.
To investigate the effect of RES on HO-1 protein expression in rat heart after different duration of hypothermic preservation.
The Langendorff model of isolated rat heart was used. After being stored in 4°C different Celsior solution for 9 h, Sprague-Dawley rats hearts were divided into 6 groups randomly: control group, 9 h group, 3 μM RES group, 10 μM RES group, 30 μM RES group, and 100 μM RES group. The morphological changes of cardiomyocytes were detected with the hematoxylin & eosin (H&E) staining using a light microscope. The mRNA and protein expression of HO-1 were detected using reverse-transcription polymerase chain reaction (RT-PCR) and western blotting.
Compared with the control group, cardiomyocytes were obviously injured in the 9 h group and the protein and mRNA expression of HO-1 were obviously decreased. Compared with the 9 h group, the mRNA and protein expression of HO-1 were increased in dose-dependent manner in the 3 μM RES, 10 μM RES and 30 μM RES group. Compared with the 9 h group, rat myocardial injury was gradually alleviated in 3 μM RES, 10 μM RES and 30 μM RES groups. However, the rat myocardial injury in the 100 μM RES group showed no more obvious improvement than in the 30 μM RES group.
In the isolated rat heart, RES protects cardiomyocytes against hypothermic preservation injury through increasing HO-1 protein expression.
白藜芦醇（RES）是一种多酚化合物和天然植物抗毒素，在各种人类疾病中发挥着潜在作用。研究证实 RES 在心脏保护中具有重要功能。
探讨 RES 对不同冷保存时间大鼠心脏 HO-1 蛋白表达的影响。
采用离体大鼠心脏 Langendorff 模型。在 4°C 不同 Celsior 溶液中保存 9 h 后，将 Sprague-Dawley 大鼠心脏随机分为 6 组：对照组、9 h 组、3 μM RES 组、10 μM RES 组、30 μM RES 组和 100 μM RES 组。用苏木精和伊红（H&E）染色法在光镜下观察心肌细胞的形态变化。采用逆转录聚合酶链反应（RT-PCR）和 Western blot 检测 HO-1 的 mRNA 和蛋白表达。
与对照组相比，9 h 组心肌细胞明显受损，HO-1 的蛋白和 mRNA 表达明显降低。与 9 h 组相比，3 μM RES、10 μM RES 和 30 μM RES 组 HO-1 的 mRNA 和蛋白表达呈剂量依赖性增加。与 9 h 组相比，3 μM RES、10 μM RES 和 30 μM RES 组大鼠心肌损伤逐渐减轻。然而，在 100 μM RES 组，大鼠心肌损伤与 30 μM RES 组相比没有明显改善。
在离体大鼠心脏中，RES 通过增加 HO-1 蛋白表达来保护心肌细胞免受低温保存损伤。
