Pagani F, Sagrada A, Brambilla A, Brunet S, Giachetti A
Department of Pharmacology, Istituto De Angeli, Milan, Italy.
Arch Int Pharmacodyn Ther. 1988 Jan-Feb;291:253-62.
Parotid saliva secretion was studied in conscious dogs in which a chronic fistula of the parotid duct was provoked by a simple surgical procedure. To validate the technique employed, the responsiveness of the gland to various stimuli was examined. The volume of secretion was measured as well as the concentrations of Na+, K+ and calcium. Secretion was elicited by administering bethanechol i.v. or by feeding a meat meal. These stimuli, applied repetitively in the same animal, evoked reproducible secretory responses. In addition, the dose-response curve to bethanechol could also be constructed. The parotid secretion is controlled by muscarinic receptor activation, as illustrated by atropine blockade and its insensitivity to adrenergic drugs. The preparation is a versatile model which allows to investigate secretion evoked by both direct and reflex activation. The advantages of the preparation, which avoids invasive techniques, are accurate measurements and reproducible responses over long periods in a conscious animal.
通过一个简单的外科手术，在清醒的犬类中引发腮腺导管慢性瘘管，以此来研究腮腺唾液分泌。为验证所采用的技术，检测了腺体对各种刺激的反应性。测量了分泌量以及钠、钾和钙的浓度。通过静脉注射氨甲酰甲胆碱或喂食肉餐来引发分泌。在同一动物中重复施加这些刺激，可诱发可重复的分泌反应。此外，还可以构建氨甲酰甲胆碱的剂量反应曲线。腮腺分泌受毒蕈碱受体激活的控制，阿托品阻断以及其对肾上腺素能药物不敏感就说明了这一点。该制备方法是一个通用模型，可用于研究直接激活和反射激活所诱发的分泌。该制备方法避免了侵入性技术，其优点是在清醒动物中能进行准确测量且能在长时间内产生可重复的反应。
