Department of pathology, General Hospital Graz II, Graz, Austria.
Institute of Biomedical Sciences, Faculty of Medicine, University of Maribor, Maribor, Slovenia.
J Cell Mol Med. 2021 Apr;25(8):3680-3698. doi: 10.1111/jcmm.16397. Epub 2021 Mar 2.
Cell lines are widely used for various research purposes including cancer and drug research. Recently, there have been studies that pointed to discrepancies in the literature and usage of cell lines. That is why we have prepared a comprehensive overview of the most common gynaecological cancer cell lines, their literature, a list of currently available cell lines, and new findings compared with the original studies. A literature review was conducted via MEDLINE, PubMed and ScienceDirect for reviews in the last 5 years to identify research and other studies related to gynaecological cancer cell lines. We present an overview of the current literature with reference to the original studies and pointed to certain inconsistencies in the literature. The adherence to culturing rulesets and the international guidelines helps in minimizing replication failure between institutions. Evidence from the latest research suggests that despite certain drawbacks, variations of cancer cell lines can also be useful in regard to a more diverse genomic landscape.
细胞系被广泛应用于各种研究目的，包括癌症和药物研究。最近，有研究指出细胞系在文献和使用方面存在差异。这就是为什么我们准备了一份最常见妇科癌症细胞系的综合概述，包括它们的文献、目前可用的细胞系列表，以及与原始研究相比的新发现。通过 MEDLINE、PubMed 和 ScienceDirect 进行了文献回顾，以查找过去 5 年内与妇科癌症细胞系相关的研究和其他研究。我们参考原始研究，概述了当前的文献，并指出文献中存在某些不一致之处。遵守培养规则和国际准则有助于最大限度地减少机构间复制失败。最新研究的证据表明，尽管存在某些缺点，但癌细胞系的变异在更广泛的基因组景观方面也可能是有用的。
