Duanghathaipornsuk Surachet, Kim Dong-Shik, Phares Tamara L, Li Cheng-Han, Jinschek Joerg R, Alba-Rubio Ana C
Department of Chemical Engineering, The University of Toledo, Toledo, OH 43606, USA.
Department of Bioengineering, The University of Toledo, Toledo, OH 43606, USA.
Nanoscale. 2021 Mar 12;13(9):5136-5144. doi: 10.1039/d1nr00015b.
It is well known that an excess of hydroxyl radicals (˙OH) in the human body is responsible for oxidative stress-related diseases. An understanding of the relationship between the concentration of ˙OH and those diseases could contribute to better diagnosis and prevention. Here we present a supersensitive nanosensor integrated with an electrochemical method to measure the concentration of ˙OH in vitro. The electrochemical sensor consists of a composite comprised of ultrasmall cerium oxide nanoclusters (<2 nm) grafted to a highly conductive carbon deposited on a screen-printed carbon electrode (SPCE). Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to analyze the interaction between cerium oxide nanoclusters and ˙OH. The CV results demonstrated that this electrochemical sensor had the capacity of detecting ˙OH with a high degree of accuracy and selectivity, achieving a consistent performance. Additionally, EIS results confirmed that our electrochemical sensor was able to differentiate ˙OH from hydrogen peroxide (H2O2), which is another common reactive oxygen species (ROS) found in the human body. The limit of detection (LOD) observed with this electrochemical sensor was of 0.6 μM. Furthermore, this nanosized cerium oxide-based electrochemical sensor successfully detected in vitro the presence of ˙OH in preosteoblast cells from newborn mouse bone tissue. The supersensitive electrochemical sensor is expected to be beneficially used in multiple applications, including medical diagnosis, fuel-cell technology, and food and cosmetic industries.
众所周知，人体内过量的羟基自由基（˙OH）会引发与氧化应激相关的疾病。了解˙OH浓度与这些疾病之间的关系有助于更好地进行诊断和预防。在此，我们展示了一种集成电化学方法的超灵敏纳米传感器，用于体外测量˙OH的浓度。该电化学传感器由一种复合材料组成，该复合材料是由接枝到沉积在丝网印刷碳电极（SPCE）上的高导电碳上的超小氧化铈纳米团簇（<2 nm）构成。采用循环伏安法（CV）和电化学阻抗谱（EIS）分析氧化铈纳米团簇与˙OH之间的相互作用。CV结果表明，这种电化学传感器具有高精度和高选择性检测˙OH的能力，性能一致。此外，EIS结果证实，我们的电化学传感器能够区分˙OH和过氧化氢（H2O2），过氧化氢是人体中另一种常见的活性氧（ROS）。该电化学传感器的检测限（LOD）为0.6 μM。此外，这种基于纳米氧化铈的电化学传感器成功地在体外检测到了新生小鼠骨组织前成骨细胞中˙OH的存在。这种超灵敏电化学传感器有望在多种应用中得到有益应用，包括医学诊断、燃料电池技术以及食品和化妆品行业。
