Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy.
Methods Mol Biol. 2021;2292:17-22. doi: 10.1007/978-1-0716-1354-2_2.
Urine cell-free DNA is an important source of diagnostic markers for different diseases, especially for cancer. It could be important to achieve the urine cell-free DNA integrity to establish its provenience from cancer cells or dead inflammatory cells for necrosis in urine or from normal cells with the purpose to use it as an early diagnostic tool for urological cancers or other diseases. Here we describe a simple, noninvasive approach from urine collection to DNA integrity analysis using real-time PCR.
尿液无细胞 DNA 是不同疾病（尤其是癌症）诊断标志物的重要来源。获得尿液无细胞 DNA 的完整性非常重要，这有助于确定其源自癌细胞或尿液中坏死的炎性细胞，还是源自正常细胞，目的是将其用作泌尿系统癌症或其他疾病的早期诊断工具。在此，我们描述了一种从尿液采集到使用实时 PCR 进行 DNA 完整性分析的简单、非侵入性方法。
