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体外IgG抗半抗原抗体分泌在大量前体细胞增殖后开始。

IgG anti-hapten antibody secretion in vitro commences after extensive precursor proliferation.

作者信息

Kemshead J T, North J R, Askonas B A

出版信息

Immunology. 1977 Oct;33(4):485-90.

PMID:336521
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1445380/
Abstract

We have investigated whether the intermediate cells that arise in primed mice after boosting require further cycles of division in culture before maturation into IgG antibody secreting cells. Killing of dividing cells between days 1--4 in culture, by exposure to BUdR-uv irradiation ablated the high IgG response observed on day 5 in control cultures. After T cell removal and replacement by a soluble factor (TRF) similar results were obtained. Thus B cell division over an extended period occurs prior to the appearance of IgG secreting cells. Furthermore, autoradiography of plaques from cultures briefly exposed to [3H]thymidine before harvest showed that some antibody secreting cells were synthesizing DNA at the time of assay.

摘要

我们研究了在加强免疫后初免小鼠中出现的中间细胞在体外培养中成熟为分泌IgG抗体的细胞之前是否需要进一步的分裂周期。通过暴露于溴脱氧尿苷-紫外线照射，在培养的第1至4天杀死正在分裂的细胞，消除了对照培养物中在第5天观察到的高IgG反应。在去除T细胞并用一种可溶性因子（TRF）替代后，得到了类似的结果。因此，在分泌IgG的细胞出现之前，B细胞会在较长时间内进行分裂。此外，在收获前短暂暴露于[3H]胸腺嘧啶核苷的培养物中斑块的放射自显影显示，一些抗体分泌细胞在检测时正在合成DNA。

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本文引用的文献

1
Cell populations and cell proliferation in the in vitro response of normal mouse spleen to heterologous erythrocytes. Analysis by the hot pulse technique.正常小鼠脾脏对异种红细胞体外反应中的细胞群体与细胞增殖。热脉冲技术分析
J Exp Med. 1967 Sep 1;126(3):443-54. doi: 10.1084/jem.126.3.443.
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Immunization of dissociated spleen cell cultures from normal mice.对来自正常小鼠的脾细胞解离培养物进行免疫接种。
J Exp Med. 1967 Sep 1;126(3):423-42. doi: 10.1084/jem.126.3.423.
3
Correlation of grain counts with radioactivity (125I and tritium) in autoradiography.放射自显影中颗粒计数与放射性（125I和氚）的相关性。
Exp Cell Res. 1966 Mar;41(3):557-72. doi: 10.1016/s0014-4827(66)80106-4.
4
Precursor cell division during the immune response in vitro: antigen-induced and "spontaneous" antibody-forming cells.体外免疫应答过程中的前体细胞分裂：抗原诱导的和“自发”的抗体形成细胞。
Eur J Immunol. 1973 Oct;3(10):655-6. doi: 10.1002/eji.1830031014.
5
Direct conversion of precursors of PFCs into active PFCs in vitro, without prior cell division.在体外将全氟化碳前体直接转化为活性全氟化碳，无需预先进行细胞分裂。
Immunology. 1973 Apr;24(4):707-10.
6
Autoradiographic studies on the proliferation of antibody-producing cells in vitro.体外抗体产生细胞增殖的放射自显影研究。
J Exp Med. 1974 Mar 1;139(3):754-60. doi: 10.1084/jem.139.3.754.
7
B lymphocyte differentiation induced by lipopolysaccharide. I. Generation of cells synthesizing four major immunoglobulin classes.脂多糖诱导的B淋巴细胞分化。I. 合成四种主要免疫球蛋白类别的细胞的产生
J Immunol. 1975 Sep;115(3):671-6.
8
IgG response in vitro. I. The requirement for an intermediate responsive cell type.体外IgG反应。I. 对中间反应性细胞类型的需求。
Eur J Immunol. 1976 Jan;6(1):8-15. doi: 10.1002/eji.1830060104.
9
Several proliferative phases precede maturation of IgG-secreting cells in mitogen-stimulated cultures.在有丝分裂原刺激的培养物中，IgG分泌细胞成熟之前有几个增殖阶段。
Nature. 1976 Apr 15;260(5552):611-3. doi: 10.1038/260611a0.

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