Kemshead J T, North J R, Askonas B A
Immunology. 1977 Oct;33(4):485-90.
We have investigated whether the intermediate cells that arise in primed mice after boosting require further cycles of division in culture before maturation into IgG antibody secreting cells. Killing of dividing cells between days 1--4 in culture, by exposure to BUdR-uv irradiation ablated the high IgG response observed on day 5 in control cultures. After T cell removal and replacement by a soluble factor (TRF) similar results were obtained. Thus B cell division over an extended period occurs prior to the appearance of IgG secreting cells. Furthermore, autoradiography of plaques from cultures briefly exposed to [3H]thymidine before harvest showed that some antibody secreting cells were synthesizing DNA at the time of assay.
我们研究了在加强免疫后初免小鼠中出现的中间细胞在体外培养中成熟为分泌IgG抗体的细胞之前是否需要进一步的分裂周期。通过暴露于溴脱氧尿苷-紫外线照射,在培养的第1至4天杀死正在分裂的细胞,消除了对照培养物中在第5天观察到的高IgG反应。在去除T细胞并用一种可溶性因子(TRF)替代后,得到了类似的结果。因此,在分泌IgG的细胞出现之前,B细胞会在较长时间内进行分裂。此外,在收获前短暂暴露于[3H]胸腺嘧啶核苷的培养物中斑块的放射自显影显示,一些抗体分泌细胞在检测时正在合成DNA。