体外抗体产生细胞增殖的放射自显影研究。
Autoradiographic studies on the proliferation of antibody-producing cells in vitro.
作者信息
Hünig T, Schimpl A, Wecker E
出版信息
J Exp Med. 1974 Mar 1;139(3):754-60. doi: 10.1084/jem.139.3.754.
Abstract
A rapid and reliable autoradiographic technique for plaque-forming cells (PFCs) using (14)C rather than tritiated thymidine is described. Its application to PFCs developing in vitro shows that (a) practically all PFCs derive from precursors dividing steadily during the culture period, (b) PFC precursors divide in the absence of T-cell helper function, and (c) at least some PFCs may continue to divide.
摘要
本文描述了一种使用¹⁴C而非氚标记胸腺嘧啶核苷的快速可靠的用于形成蚀斑细胞（PFC）的放射自显影技术。将其应用于体外发育的PFC表明：（a）实际上所有PFC均源自培养期间持续分裂的前体细胞；（b）PFC前体细胞在没有T细胞辅助功能的情况下进行分裂；（c）至少一些PFC可能会继续分裂。
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本文引用的文献
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Autoradiographic studies on the immune response.I. The kinetics of plasma cell proliferation.免疫反应的放射自显影研究。I. 浆细胞增殖的动力学
J Exp Med. 1962 Jan 1;115(1):209-30. doi: 10.1084/jem.115.1.209.
2
Autoradiographic studies on the immune response. II. DNA synthesis amongst single antibody-producing cells.免疫反应的放射自显影研究。II. 单个抗体产生细胞中的DNA合成。
J Exp Med. 1962 Jan 1;115(1):231-44. doi: 10.1084/jem.115.1.231.
3
Plaque formation in agar by single antibody-producing cells.单个产生抗体的细胞在琼脂中形成菌斑。
Science. 1963 Apr 26;140(3565):405.
4
Cell populations and cell proliferation in the in vitro response of normal mouse spleen to heterologous erythrocytes. Analysis by the hot pulse technique.正常小鼠脾脏对异种红细胞体外反应中的细胞群体与细胞增殖。热脉冲技术分析
J Exp Med. 1967 Sep 1;126(3):443-54. doi: 10.1084/jem.126.3.443.
5
Immunization of dissociated spleen cell cultures from normal mice.对来自正常小鼠的脾细胞解离培养物进行免疫接种。
J Exp Med. 1967 Sep 1;126(3):423-42. doi: 10.1084/jem.126.3.423.
6
7
Inhibition of in vitro immune response by treatment of spleen cell suspensions with anti-theta serum.用抗θ血清处理脾细胞悬液对体外免疫反应的抑制作用。
Nature. 1970 Jun 27;226(5252):1258-9. doi: 10.1038/2261258a0.
8
Direct conversion of precursors of PFCs into active PFCs in vitro, without prior cell division.在体外将全氟化碳前体直接转化为活性全氟化碳，无需预先进行细胞分裂。
Immunology. 1973 Apr;24(4):707-10.
9
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