Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, China.
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
Food Chem. 2021 Aug 1;352:129312. doi: 10.1016/j.foodchem.2021.129312. Epub 2021 Feb 20.
High-value acacia honey is often adulterated with inexpensive high fructose corn syrup (HFCS), due to their similar color and sugar composition. α‑Dicarbonyl compounds formed by Maillard reaction or caramelization during heat treatment or storage, differ between HFCS and honey due to differences in starting materials and processing methods. In this study, we compared α-dicarbonyl compounds in acacia honey and HFCS by Ion Mobility-Mass Spectrometry and multivariate statistical analysis. Through α-dicarbonyl compound derivatization with o-phenylenediamine, we screened a marker with 189.1023 m/z and 139.3 Å Collision Cross-Section that can distinguish HFCS from acacia honey. Nuclear magnetic resonance spectra identified this marker compound as 3,4-dideoxypentosulose. We then used chromatography-coupled tandem mass spectrometry to quantitate 3,4-dideoxypentosulose in market samples of honey and HFCS and found that 3,4-dideoxypentosulose was negligible (<0.098 mg/kg) in honey, but prevalent in HFCS (≧1.174 mg/kg), indicating 3,4-dideoxypentosulose can serve as an alternative indicator of HFCS adulteration of acacia honey.
高价值的阿拉伯胶蜂蜜常因颜色和糖组成与廉价的高果糖玉米糖浆 (HFCS) 相似而被掺假。由于起始原料和加工方法的不同，美拉德反应或热处理或储存过程中形成的焦糖 α-二羰基化合物在 HFCS 和蜂蜜之间存在差异。在这项研究中，我们通过离子淌度-质谱联用和多变量统计分析比较了阿拉伯胶蜂蜜和 HFCS 中的 α-二羰基化合物。通过与邻苯二胺对 α-二羰基化合物进行衍生化，我们筛选出了一个具有 189.1023 m/z 和 139.3 Å 碰撞截面的标志物，可将 HFCS 与阿拉伯胶蜂蜜区分开来。核磁共振光谱鉴定出该标志物化合物为 3,4-二脱氧戊糖。然后，我们使用色谱-串联质谱法对蜂蜜和 HFCS 市售样品中的 3,4-二脱氧戊糖进行定量，发现 3,4-二脱氧戊糖在蜂蜜中可忽略不计（<0.098 mg/kg），但在 HFCS 中普遍存在（≧1.174 mg/kg），表明 3,4-二脱氧戊糖可作为 HFCS 掺假阿拉伯胶蜂蜜的替代指标。
