Martinello Marianna, Stella Roberto, Baggio Alessandra, Biancotto Giancarlo, Mutinelli Franco
National Reference Laboratory for Honey Bee Health, Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy.
Department of Chemistry, Istituto Zooprofilattico Sperimentale delle Venezie, 35020 Legnaro, Italy.
Metabolites. 2022 Oct 18;12(10):985. doi: 10.3390/metabo12100985.
Honey is a natural product that is in great demand and has a relatively high price, thus making it one of the most common targets of economically motivated adulteration. Its adulteration can be obtained by adding cheaper honey or sugar syrups or by overfeeding honeybees with sugar syrups. Adulteration techniques are constantly evolving and advanced techniques and instruments are required for its detection. We used non-targeted metabolomics to underscore potential markers of honey adulteration with sugar syrups. The metabolomic profiles of unadulterated honeys and sugar beet, corn and wheat syrups were obtained using hydrophilic interaction liquid chromatography high-resolution mass spectrometry (LC-HRMS). The potential markers have been selected after data processing. Fortified honey (5%, 10% and 20%), honey obtained from overfeeding, and 58 commercial honeys were analyzed. One potential marker appeared with a specific signal for syrups and not for honey. This targeted analysis showed a linear trend in fortified honeys with a calculated limit of quantification around 5% of fortification.
蜂蜜是一种需求量很大且价格相对较高的天然产品，因此成为出于经济动机掺假的最常见目标之一。其掺假方式可以是添加更便宜的蜂蜜或糖浆，或者用糖浆过度喂养蜜蜂。掺假技术不断演变，需要先进的技术和仪器来进行检测。我们使用非靶向代谢组学来强调蜂蜜被糖浆掺假的潜在标志物。使用亲水相互作用液相色谱高分辨率质谱（LC-HRMS）获得了未掺假蜂蜜以及甜菜、玉米和小麦糖浆的代谢组学图谱。在数据处理后选择了潜在标志物。对加标蜂蜜（5%、10%和20%）、过度喂养得到的蜂蜜以及58种市售蜂蜜进行了分析。一种潜在标志物出现了糖浆特有的信号，而不是蜂蜜的信号。这种靶向分析显示加标蜂蜜呈线性趋势，计算出的定量限约为加标量的5%。
