Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, GSP-1, Moscow 119991, Russia.
Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, GSP-1, Moscow 119991, Russia.
J Chromatogr A. 2021 Apr 12;1642:462010. doi: 10.1016/j.chroma.2021.462010. Epub 2021 Feb 18.
Evaluation of the chromatographic properties of covalently bonded hyperbranched stationary phase based on poly(styrene-divinylbenzene) (PS-DVB) and containing zwitterionic fragments in the structure of functional layer was conducted in suppressed ion chromatography (IC), reversed phase high performance liquid chromatography (RP HPLC), and hydrophilic interaction liquid chromatography (HILIC) modes. Besides the possibility of resolving 20 inorganic anions and organic acids using KOH eluent in suppressed IC, prepared resin provided the separation of alkylbenzenes in RP HPLC, water-soluble vitamins, amino acids, and sugars in HILIC mode. Trends in the retention of hydrophobic and polar analytes on the prepared stationary phase indicated the dominating effect of analyte nature on the retention mechanism and proved satisfactory hydrophilization of PS-DVB surface with hyperbranched functional layer for retaining polar compounds. The obtained results revealed good prospects of using hydrophobic PS-DVB substrate for preparing stationary phases for mixed-mode chromatography.
评价了化学键合超支化固定相的色谱性能，该固定相基于聚（苯乙烯-二乙烯基苯）（PS-DVB），在功能层的结构中含有两性离子片段，分别在抑制离子色谱（IC）、反相高效液相色谱（RP HPLC）和亲水相互作用液相色谱（HILIC）模式下进行。除了使用 KOH 洗脱剂在抑制 IC 中分离 20 种无机阴离子和有机酸的可能性外，所制备的树脂还在 RP HPLC 中提供了烷基苯的分离，在 HILIC 模式下提供了水溶性维生素、氨基酸和糖的分离。疏水性和亲水性分析物在制备好的固定相上的保留趋势表明，分析物性质对保留机制具有主导作用，并证明了超支化功能层对 PS-DVB 表面的良好亲水化作用，有利于保留极性化合物。所得结果表明，利用疏水性 PS-DVB 基质制备混合模式色谱用固定相具有良好的应用前景。
