Peng Yahui, Hou Yanjie, Zhang Feifang, Shen Guobin, Yang Bingcheng
Pharmacy School, East China University of Science and Technology, 267 BO, 130 Meilong Road, Shanghai, 200237, China.
Anal Bioanal Chem. 2016 May;408(13):3633-8. doi: 10.1007/s00216-016-9446-7. Epub 2016 Mar 12.
A hyperbranched stationary phase for hydrophilic interaction liquid chromatography (HILIC) has been prepared by grafting polyethylenimine (PEI) onto silica gel (termed as PEI-Sil). Rich primary, secondary, and tertiary amino groups associated with PEI render its good hydrophility. More importantly, the hyperbranched structure of PEI molecule is greatly helpful in improving interaction with polar analytes. For several kinds of model polar compounds, including organic acids, nucleosides, nucleic acid bases, amino acids, cephalosporins, and non-reducing sugars, PEI-Sil demonstrated excellent separation performance in terms of running stability, reproducibility, and separation efficiency (e.g., plate count ~74,000/m). In addition, PEI-Sil also exhibited much better separation selectivity toward inorganic anions when operated in the mode of ion chromatography relative to a commercial amino propyl-bonded column.
通过将聚乙烯亚胺（PEI）接枝到硅胶上（称为PEI-Sil），制备了一种用于亲水作用液相色谱（HILIC）的超支化固定相。与PEI相关的丰富的伯、仲和叔氨基使其具有良好的亲水性。更重要的是，PEI分子的超支化结构极大地有助于改善与极性分析物的相互作用。对于几种模型极性化合物，包括有机酸、核苷、核酸碱基、氨基酸、头孢菌素和非还原糖，PEI-Sil在运行稳定性、重现性和分离效率方面（例如，塔板数~74,000/m）表现出优异的分离性能。此外，相对于商业氨基丙基键合柱，当以离子色谱模式操作时，PEI-Sil对无机阴离子也表现出更好的分离选择性。
