Hustmyer F G, Nonnecke B J, Beitz D C, Horst R L, Reinhardt T A
National Animal Disease Center, U.S. Department of Agriculture, Ames, IA.
Biochem Biophys Res Commun. 1988 Apr 29;152(2):545-51. doi: 10.1016/s0006-291x(88)80072-x.
The effect of 1,25-(OH)2D3 on Concanavalin-A (ConA) mediated bovine lymphocyte proliferation was dependent on the magnitude of the proliferative response. Lymphocytes from cows with high inherent proliferative responses (134,904 +/- 15,488 cpm/well) were unaffected by 1,25-(OH)2D3; whereas, lymphocytes from cows with low inherent proliferative responses (46,317 +/- 6000 cpm/well) were stimulated 75% (P less than 0.01) by 10(-10)M 1,25-(OH)2D3. At low initial proliferation rates, seen with low density cultures enriched for lymphocytes, the effect of 1,25-(OH)2D3 on proliferation changed from inhibition in the absence of added monocytes to stimulation at 18-23% monocyte addition. These results suggest that 1,25-(OH)2D3 possesses both stimulatory and inhibitory effects on ConA-induced proliferation that are monocyte dependent.
1,25 -（OH）₂D₃对刀豆球蛋白A（ConA）介导的牛淋巴细胞增殖的影响取决于增殖反应的强度。来自具有高固有增殖反应（134,904±15,488 cpm/孔）的奶牛的淋巴细胞不受1,25 -（OH）₂D₃的影响；而来自具有低固有增殖反应（46,317±6000 cpm/孔）的奶牛的淋巴细胞受到10⁻¹⁰M 1,25 -（OH）₂D₃刺激，增殖提高了75%（P＜0.01）。在低密度培养且富含淋巴细胞时出现的低初始增殖率情况下，1,25 -（OH）₂D₃对增殖的影响从在不添加单核细胞时的抑制作用转变为添加18 - 23%单核细胞时的刺激作用。这些结果表明，1,25 -（OH）₂D₃对ConA诱导的增殖具有刺激和抑制作用，且这些作用依赖于单核细胞。
