• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Rapid isolation of lysyl-tRNA synthetase from rat liver.

作者信息

Kumar A M, Nayak R

机构信息

Microbiology and Cell Biology Laboratory, Indian Institute of Science, Bangalore.

出版信息

Biochem Biophys Res Commun. 1988 Apr 29;152(2):593-7. doi: 10.1016/s0006-291x(88)80079-2.

DOI:10.1016/s0006-291x(88)80079-2
PMID:3365243
Abstract

The high molecular weight aminoacyl-tRNA synthetase complex (the 24S complex) was isolated from rat liver by ultracentrifugation. The lysyl-tRNA synthetase (E.C. 6.1.1.6) was selectively dissociated by hydrophobic interaction chromatography on 1,6 diaminohexyl agarose followed by hydroxylapatite chromatography and DEAE chromatography. The lysyl-tRNA synthetase dissociated from the 24S synthetase complex was purified approximately to 2700 fold with 14% yield.

摘要

相似文献

1
Rapid isolation of lysyl-tRNA synthetase from rat liver.
Biochem Biophys Res Commun. 1988 Apr 29;152(2):593-7. doi: 10.1016/s0006-291x(88)80079-2.
2
Purification and characterization of lysyl-tRNA synthetase after dissociation of the particulate aminoacyl-tRNA synthetases from rat liver.大鼠肝脏中颗粒状氨酰-tRNA合成酶解离后赖氨酰-tRNA合成酶的纯化与特性分析
J Biol Chem. 1980 May 10;255(9):4362-6.
3
Multiple forms of arginyl- and lysyl-tRNA synthetases in rat liver: a re-evaluation.大鼠肝脏中精氨酰-tRNA合成酶和赖氨酰-tRNA合成酶的多种形式：重新评估
Biochim Biophys Acta. 1985 Jun 10;829(2):173-9. doi: 10.1016/0167-4838(85)90186-4.
4
[A new method of isolating lysyl-tRNA-synthetase from the rat liver].
Ukr Biokhim Zh (1978). 1986 May-Jun;58(3):58-61.
5
Physical and biochemical characterization of a purified arginyl-tRNA synthetase-lysyl-tRNA-synthetase complex from rat liver.来自大鼠肝脏的纯化精氨酰 - tRNA合成酶 - 赖氨酰 - tRNA合成酶复合物的物理和生化特性
Biochemistry. 1982 Apr 13;21(8):1959-66. doi: 10.1021/bi00537a040.
6
Leucyl-tRNA and lysyl-tRNA synthetases, derived from the high-Mr complex of sheep liver, are hydrophobic proteins.
Eur J Biochem. 1985 Aug 15;151(1):101-10. doi: 10.1111/j.1432-1033.1985.tb09074.x.
7
Comparison of the enzymatic behavior of high molecular weight and free lysyl-tRNA synthetase from rat liver: kinetic analysis of lysylation of tRNA.
Arch Biochem Biophys. 1986 Sep;249(2):407-17. doi: 10.1016/0003-9861(86)90017-2.
8
[Isolation and properties of lysyl-tRNA-synthetase from rabbit skeletal muscles].
Ukr Biokhim Zh (1978). 1988 Jul-Aug;60(4):29-34.
9
Polypeptide composition of the 8S form of prolyl-tRNA synthetase from rat liver.
Biochem Biophys Res Commun. 1992 Sep 16;187(2):1071-6. doi: 10.1016/0006-291x(92)91306-b.
10
Multiple molecular forms of cysteinyl-tRNA synthetase from rat liver: purification and subunit structure.大鼠肝脏中半胱氨酰 - tRNA合成酶的多种分子形式：纯化及亚基结构
Biochim Biophys Acta. 1976 Nov 8;452(1):271-83. doi: 10.1016/0005-2744(76)90080-2.