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[A new method of isolating lysyl-tRNA-synthetase from the rat liver].

作者信息

Demchenko I B, Kucherenko N E, Vinogradova R P, Sleptsova I L

出版信息

Ukr Biokhim Zh (1978). 1986 May-Jun;58(3):58-61.

PMID:3727036
Abstract

The methods of gel filtration on sepharose 6B, hydrophobic chromatography, chromatofocusing were used to isolate the preparation of lysyl-tRNA-synthetase. The enzyme activity at the terminal stage of purification is 1800 times as high. The isolated preparation is homogeneous with electrophoresis in 4-30% PAAG.

摘要

相似文献

1
[A new method of isolating lysyl-tRNA-synthetase from the rat liver].
Ukr Biokhim Zh (1978). 1986 May-Jun;58(3):58-61.
2
Rapid isolation of lysyl-tRNA synthetase from rat liver.
Biochem Biophys Res Commun. 1988 Apr 29;152(2):593-7. doi: 10.1016/s0006-291x(88)80079-2.
3
Purification and characterization of lysyl-tRNA synthetase after dissociation of the particulate aminoacyl-tRNA synthetases from rat liver.大鼠肝脏中颗粒状氨酰-tRNA合成酶解离后赖氨酰-tRNA合成酶的纯化与特性分析
J Biol Chem. 1980 May 10;255(9):4362-6.
4
Physical and biochemical characterization of a purified arginyl-tRNA synthetase-lysyl-tRNA-synthetase complex from rat liver.来自大鼠肝脏的纯化精氨酰 - tRNA合成酶 - 赖氨酰 - tRNA合成酶复合物的物理和生化特性
Biochemistry. 1982 Apr 13;21(8):1959-66. doi: 10.1021/bi00537a040.
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[Isolation and properties of lysyl-tRNA-synthetase from rabbit skeletal muscles].
Ukr Biokhim Zh (1978). 1988 Jul-Aug;60(4):29-34.
6
Leucyl-tRNA and lysyl-tRNA synthetases, derived from the high-Mr complex of sheep liver, are hydrophobic proteins.
Eur J Biochem. 1985 Aug 15;151(1):101-10. doi: 10.1111/j.1432-1033.1985.tb09074.x.
7
Multiple forms of arginyl- and lysyl-tRNA synthetases in rat liver: a re-evaluation.大鼠肝脏中精氨酰-tRNA合成酶和赖氨酰-tRNA合成酶的多种形式:重新评估
Biochim Biophys Acta. 1985 Jun 10;829(2):173-9. doi: 10.1016/0167-4838(85)90186-4.
8
Affinity chromatography of rat liver aminoacyl-tRNA synthetase complex.
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9
[Physico-chemical properties of lysyl-tRNA-synthetase from the rat liver].[大鼠肝脏赖氨酰 - tRNA合成酶的物理化学性质]
Ukr Biokhim Zh (1978). 1985 Jul-Aug;57(4):19-23.
10
Multiple molecular forms of cysteinyl-tRNA synthetase from rat liver: purification and subunit structure.大鼠肝脏中半胱氨酰 - tRNA合成酶的多种分子形式:纯化及亚基结构
Biochim Biophys Acta. 1976 Nov 8;452(1):271-83. doi: 10.1016/0005-2744(76)90080-2.