液基细胞学与传统制片在子宫内膜样腺癌Ⅰ级和Ⅲ级中的形态学差异，以及两种方法中分级的差异。

Morphological Differences between Liquid-Based Cytology and Conventional Preparation in Endometrial Endometrioid Carcinoma Grade 1 and Grade 3, and the Differentiation of Grades in Each Method.

机构信息

Department of Bioscience and Laboratory Medicine, Hirosaki University Graduate School of Health Sciences, Aomori, Japan.

Anatomic Pathology Laboratory, Hirosaki University l Hospital, Aomori, Japan.

出版信息

Acta Cytol. 2021;65(3):227-234. doi: 10.1159/000512867. Epub 2021 Mar 2.

DOI:10.1159/000512867

PMID:33652432

Abstract

INTRODUCTION

Direct smearing preparation (conventional preparation [CP]) has been widely used for endometrial cytology in Japan. In CP, sampling and screening errors are problematic. In liquid-based cytology preparation (LBC), the problems of CP can be solved. But there is a problem that cytological findings of LBC are different from those of CP. The purpose of this study was to evaluate the differences of morphological findings of endometrial cytology between LBC and CP, and the usefulness of the endometrial LBC to differentiate endometrioid carcinoma grade 1 (G1) from grade 3 (G3).

METHODS

Thirteen cases of endometrioid carcinoma G1, and 5 cases of G3 collected by the Softcyte device and prepared by LBC and CP (split specimen) were used. We focused on the following items: (1) the number of clusters per cm2, (2) the number of layers of clusters, (3) area of clusters, (4) perimeter of clusters, (5) roundness of clusters, (6) complexity of clusters, (7) area of nucleus, (8) perimeter of nucleus, (9) roundness of nucleus, (10) complexity of nucleus, (11) area of nucleolus, and (12) nucleolus-nucleus ratio (N/N).

RESULTS

Compared with CP, the number of clusters and layers of the clusters in LBC were significantly larger in G1. The area and perimeters of the clusters and the nucleus were significant smaller, and the N/N ratio was greater in LBC than that in CP in both G1 and G3. Regarding morphological differences between G1 and G3 in LBC and CP, the number of layers was significantly larger in G1 than in G3 in LBC and CP. The area of the clusters in LBC was significantly larger in G1 than in G3. The area and perimeters of the nucleus in CP and the area of the nucleolus and N/N ratio in LBC and CP were significantly smaller in G1 than in G3.

CONCLUSION

In the endometrial cytology, it became clear that the cell image was different between LBC and CP and between G1 and G3. By microscopic examination understanding the characteristics of the cell image in LBC, endometrial LBC could be useful to diagnose endometrial carcinoma.

摘要

简介

直接涂片制备（常规制备 [CP]）已在日本广泛用于子宫内膜细胞学检查。在 CP 中，采样和筛选错误是有问题的。在液基细胞学制备（LBC）中，可以解决 CP 的问题。但存在 LBC 的细胞学发现与 CP 不同的问题。本研究旨在评估 LBC 和 CP 之间子宫内膜细胞学形态学发现的差异，以及子宫内膜 LBC 区分子宫内膜样癌 1 级（G1）和 3 级（G3）的有用性。

方法

使用 Softcyte 装置收集的 13 例子宫内膜样癌 G1 病例和 5 例 G3 病例，并通过 LBC 和 CP（分裂标本）进行制备。我们重点关注以下项目：（1）每平方厘米的簇数，（2）簇的层数，（3）簇的面积，（4）簇的周长，（5）簇的圆形度，（6）簇的复杂性，（7）核的面积，（8）核的周长，（9）核的圆形度，（10）核的复杂性，（11）核仁的面积，（12）核仁-核比（N/N）。

结果

与 CP 相比，LBC 中 G1 的簇数和层数明显更大。LBC 中簇和核的面积和周长明显较小，N/N 比值较大，G1 和 G3 中均如此。在 LBC 和 CP 中，G1 和 G3 之间的形态学差异方面，LBC 中 G1 的层数明显大于 G3。LBC 中 G1 的簇面积明显大于 G3。CP 中核的面积和周长以及 LBC 和 CP 中核仁的面积和 N/N 比值在 G1 中明显小于 G3。