Salvetti Elisa, Campedelli Ilenia, Larini Ilaria, Conedera Giada, Torriani Sandra
Department of Biotechnology, University of Verona, 37134 Verona, Italy.
Microbion srl, San Giovanni Lupatoto, 37057 Verona, Italy.
Microorganisms. 2021 Feb 26;9(3):491. doi: 10.3390/microorganisms9030491.
spp. are environmental microorganisms commonly associated with fermented foods. Absence of antibiotic resistance (AR) in bacteria is a critical issue for global food safety. Herein, we updated the occurrence of AR genes in the genus through in silico analyses of the genomes of 17 type strains. A total of 131 putative AR traits associated with the main clinically relevant antibiotics were detected. We found, for the first time, the A gene in ATCC 700006 and NCDO 768. Their amino acid sequences displayed high similarities (59.07% and 52.21%) with LsaA of V583, involved in clindamycin (CLI) and quinupristin-dalfopristin (QUD) resistance. This trait has different distribution patterns in nontype strains-i.e., , and isolates from fermented vegetables, cheeses, and starters. To better explore the role of A, MIC for CLI and QUD were assessed in ATCC 700006 and NCDO 768; both strains were resistant towards CLI, potentially linking A to their resistant phenotype. Contrarily, NCDO 768 was sensitive towards QUD; however, expression of A increased in presence of this antibiotic, indicating an active involvement of this trait and thus suggesting a revision of the QUD thresholds for this species.
某些物种是通常与发酵食品相关的环境微生物。细菌中不存在抗生素抗性（AR）是全球食品安全的一个关键问题。在此，我们通过对17株模式菌株的基因组进行计算机分析，更新了该属中AR基因的出现情况。共检测到131个与主要临床相关抗生素相关的推定AR特征。我们首次在ATCC 700006和NCDO 768中发现了A基因。它们的氨基酸序列与参与克林霉素（CLI）和奎奴普丁 - 达福普汀（QUD）抗性的V583的LsaA显示出高度相似性（分别为59.07%和52.21%）。该特征在非模式菌株中具有不同的分布模式，即来自发酵蔬菜、奶酪和发酵剂的某些分离株。为了更好地探究A的作用，我们评估了ATCC 700006和NCDO 768对CLI和QUD的最低抑菌浓度（MIC）；这两种菌株对CLI均有抗性，这可能将A与其抗性表型联系起来。相反，NCDO 768对QUD敏感；然而，在这种抗生素存在的情况下A的表达增加，表明该特征的积极参与，因此建议修订该物种对QUD的阈值。
