Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Rd., Guishan Dist., Taoyuan City 333, Taiwan, ROC.
Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute, 331 Shih-Pin Rd., Hsinchu 30062, Taiwan, ROC.
Int J Syst Evol Microbiol. 2020 Mar;70(3):1585-1590. doi: 10.1099/ijsem.0.003938.
A novel lactic acid bacterium, strain MB7, was isolated from lychee in Taiwan. MB7 is Gram-staining-positive, catalase-negative, non-motile, non-haemolytic, facultatively anaerobic, coccoid-shaped, heterofermentative and mainly produces d-lactic acid from glucose. Comparative analysis of 16S rRNA, and gene sequences has demonstrated that the novel strain represented a member of the genus . 16S rRNA gene sequencing results indicated that MB7 had the same sequence similarity of 99.25 % to four type strains of members of the genus : subsp. DSM 20484, subsp. DRC 1506, subsp. ATCC 8293 and DSM 20241. Additionally, high 16S rRNA sequence similarities were also observed with subsp. ATCC 19254 (99.12 %) and NRIC 1777 (98.69 %). When comparing the genomes of these type strains, the average nucleotide identity values and digital DNA-DNA hybridization values of MB7 with these type strains were 76.57-80.53 and 22.0-22.6 %, respectively. MB7 also showed different phenotypic characteristics to other most closely related species of the genus , such as carbohydrate metabolizing ability, halotolerance and growth at various pHs. On the basis of phenotypic and genotypic properties, strain MB7 represents a novel species belonging to the genus , for which the name sp. nov. is proposed. The type strain is MB7 (=BCRC 81077=NBRC 113542).
一株从台湾荔枝中分离得到的新型乳酸菌菌株 MB7 被鉴定为。MB7 革兰氏阳性，过氧化氢酶阴性，不运动，非溶血，兼性厌氧，球形，异型发酵，主要从葡萄糖产生 d-乳酸。16S rRNA 和基因序列的比较分析表明，该新型菌株代表了属的一个成员。16S rRNA 基因测序结果表明，MB7 与属内四个种的模式菌株具有相同的序列相似性：亚种。DSM 20484、亚种。DRC 1506、亚种。ATCC 8293 和 DSM 20241 的相似性为 99.25%。此外，与亚种。ATCC 19254（99.12%）和 NRIC 1777（98.69%）也有较高的 16S rRNA 序列相似性。当比较这些模式菌株的基因组时，MB7 与这些模式菌株的平均核苷酸同一性值和数字 DNA-DNA 杂交值分别为 76.57-80.53%和 22.0-22.6%。MB7 还表现出与属内其他亲缘关系最密切的种不同的表型特征，如碳水化合物代谢能力、耐盐性和在不同 pH 值下的生长。基于表型和基因型特性，菌株 MB7 代表了一个属于属的新型物种，提议将其命名为。该模式菌株为 MB7（=BCRC 81077=NBRC 113542）。
