Oral Technology, University Hospital Bonn, 53111 Bonn, Germany.
Molecules. 2021 Feb 26;26(5):1276. doi: 10.3390/molecules26051276.
Although several natural plants and mixtures have been known and used over the centuries for their antibacterial activity, few have been thoroughly explored in the field of dentistry. Thus, the aim of this study was to enhance the antimicrobial activity of a conventional glass ionomer cement (GIC) with natural plant extracts. The effect of this alteration on the bond strength and film thickness of glass ionomer cement was evaluated and related to an 0.5% chlorohexidine modified GIC. Olive leaves , Fig tree (, and the leaves and roots of Miswak ( were used to prepare an alcoholic extract mixture. The prepared extract mixture after the evaporation of the solvent was used to modify a freeze-dried glass ionomer cement at three different extracts: water mass ratios 1:2, 1:1, and 2:1. An 0.5% chlorhexidine diacetate powder was added to a conventional GIC for the preparation of a positive control group (CHX-GIC) for comparison. The bond strength to dentine was assessed using a material-testing machine at a cross head speed of 0.5 mm/min. Failure mode was analyzed using a stereomicroscope at 12× magnification. The cement film thickness was evaluated in accordance with ISO standard 9917-1. The minimum number of samples in each group was = 10. Statistical analysis was performed using a Kruskal-Wallis test followed by Dunn's post hoc test for pairwise comparison. There was a statistically insignificant difference between the median shear bond strength ( = 0.046) of the control group (M = 3.4 MPa), and each of the CHX-GIC (M = 1.7 MPa), and the three plant modified groups of 1:2, 1:1, 2:1 (M = 5.1, 3.2, and 4.3 MPa, respectively). The CHX-GIC group showed statistically significant lower median values compared to the three plant-modified groups. Mixed and cohesive failure modes were predominant among all the tested groups. All the tested groups ( < 0.001) met the ISO standard of having less than 25 µm film thickness, with the 2:1 group (M = 24 µm) being statistically the highest among all the other groups. The plant extracts did not alter either the shear bond strength or the film thickness of the GIC and thus might represent a promising additive to GICs.
虽然几个天然植物和混合物已经被认识和使用了几个世纪，因其抗菌活性，但很少有在牙科领域得到彻底的探索。因此，本研究的目的是增强常规玻璃离子水门汀（GIC）的抗菌活性与天然植物提取物。这种改变对玻璃离子水门汀的粘结强度和膜厚度的影响，并与 0.5%洗必泰改性 GIC 相关联。橄榄叶，无花果树（，和的叶子和根 Miswak（被用来制备醇提取物混合物。经蒸发溶剂后的提取物混合物被用来修饰在三个不同的提取物：水的质量比 1:2、1:1 和 2:1 冻干的玻璃离子水门汀。0.5%洗必泰二醋酸盐粉末被添加到常规 GIC 制备阳性对照组（CHX-GIC）进行比较。用材料试验机以 0.5 毫米/分钟的十字头速度评估对牙本质的粘结强度。使用立体显微镜在 12×放大率下分析失效模式。根据 ISO 标准 9917-1 评估水泥膜厚度。每组的最小样本数为 n = 10。使用 Kruskal-Wallis 检验进行统计分析，然后使用 Dunn 事后检验进行两两比较。对照组的中位剪切粘结强度（ = 0.046）（M = 3.4 MPa），CHX-GIC 组（M = 1.7 MPa）和三个植物改性组 1:2、1:1、2:1（M = 5.1、3.2 和 4.3 MPa）之间无统计学差异。CHX-GIC 组的中位值明显低于三组植物改性组。混合和内聚失效模式在所有测试组中占主导地位。所有测试组（ < 0.001）均符合 ISO 标准，膜厚度小于 25 µm，其中 2:1 组（M = 24 µm）在所有其他组中统计学上最高。植物提取物既没有改变 GIC 的剪切粘结强度也没有改变其膜厚度，因此可能是 GIC 的一种有前途的添加剂。
