Department of Orthodontics and Institute of Craniofacial Deformity, Yonsei University College of Dentistry, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
Institute of Craniofacial Deformity, Yonsei University College of Dentistry, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
Sci Rep. 2023 Mar 3;13(1):3589. doi: 10.1038/s41598-023-30670-4.
Biofilms induce microbial-mediated surface roughening and deterioration of cement. In this study, zwitterionic derivatives (ZD) of sulfobetaine methacrylate (SBMA) and 2-methacryloyloxyethyl phosphorylcholine, were added in concentrations of 0, 1, and 3% to three different types of commercially available resin-modified glass ionomer cement (RMGIC) (RMC-I: RelyX Luting 2, RMC-II: Nexus RMGI, and RMC-III: GC FujiCEM 2). The unmodified RMGICs served as the control group for comparison. The resistance of Streptococcus mutans to ZD-modified RMGIC was evaluated with a monoculture biofilm assay. The following physical properties of the ZD-modified RMGIC were assessed: wettability, film thickness, flexural strength, elastic modulus, shear bond strength, and failure mode. The ZD-modified RMGIC significantly inhibited biofilm formation, with at least a 30% reduction compared to the control group. The addition of ZD improved the wettability of RMGIC; however, only 3% of the SBMA group was statistically different (P < 0.05). The film thickness increased in proportion to the increasing ZD concentrations; there was no statistical difference within the RMC-I (P > 0.05). The experimental groups' flexural strength, elastic modulus, and shear bond strength showed an insignificant decrease from the control group; there was no statistical difference within the RMC-I (P > 0.05). The mode of failure differed slightly in each group, but all groups showed dominance in the adhesive and mixed failure. Thus, the addition of 1 wt.% ZD in RMGIC favorably enhanced the resistance to Streptococcus mutans without any tangible loss in flexural and shear bond strength.
生物膜会诱导微生物介导的表面粗化和水泥恶化。在这项研究中，两性离子衍生物（ZD）的磺基甜菜碱甲基丙烯酸酯（SBMA）和 2-甲基丙烯酰氧基乙基磷酸胆碱，分别以 0、1 和 3%的浓度添加到三种不同类型的市售树脂改性玻璃离子水泥（RMGIC）（RMC-I：RelyX 粘固粉 2、RMC-II：Nexus RMGI 和 RMC-III：GC FujiCEM 2）中。未改性的 RMGIC 用作比较的对照组。采用单培养生物膜试验评估变形链球菌对 ZD 改性 RMGIC 的抗性。评估了 ZD 改性 RMGIC 的以下物理性能：润湿性、膜厚度、弯曲强度、弹性模量、剪切结合强度和失效模式。ZD 改性 RMGIC 显著抑制生物膜形成，与对照组相比至少减少了 30%。添加 ZD 可改善 RMGIC 的润湿性；然而，只有 3%的 SBMA 组在统计学上有差异（P<0.05）。膜厚度与 ZD 浓度成正比增加；在 RMC-I 中没有统计学差异（P>0.05）。实验组的弯曲强度、弹性模量和剪切结合强度与对照组相比略有下降，但在 RMC-I 中没有统计学差异（P>0.05）。各组的失效模式略有不同，但所有组均以黏附性和混合性失效为主。因此，在 RMGIC 中添加 1wt.%的 ZD 可以有利地增强对变形链球菌的抵抗力，而弯曲强度和剪切结合强度没有明显损失。
