Nanoimmunotech, S.L. Edificio CITEXVI Fonte das Abelleiras s/n, Campus Universitario de Vigo, 36310 Vigo, Pontevedra, Spain.
J Nanosci Nanotechnol. 2021 May 1;21(5):2834-2840. doi: 10.1166/jnn.2021.19057.
Antibody-functionalized gold nanoparticle constitutes a powerful interface biosystem for biomedical applications where the properties of gold nanoparticles and the specificity of antibody-antigen interactions are combined. This study provides insight into the key factors for the development of antibody functionalized gold nanoparticles focusing on the immobilization of the antibody. Here, we address an oriented antibody immobilization procedure on gold nanoparticles. It comprises chelatemodified gold nanoparticles that are designed for oriented immobilization of IgG antibodies (end on spatial orientation) through the metal-chelation to histidine-rich metal binding site in the heavy chain (Fc) of the antibody.
抗体功能化的金纳米粒子构成了一个强大的界面生物系统，适用于结合了金纳米粒子的特性和抗体-抗原相互作用的特异性的生物医学应用。本研究深入了解了抗体功能化金纳米粒子发展的关键因素，重点是抗体的固定化。在这里，我们提出了一种在金纳米粒子上进行定向抗体固定化的方法。该方法包括螯合修饰的金纳米粒子，这些纳米粒子通过金属螯合作用与抗体重链（Fc）中富含组氨酸的金属结合位点结合，设计用于 IgG 抗体（空间取向为端到端）的定向固定化。
